an-efficient-fast-method-for-labeling-analyzing-mouse

An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli

The glomeruli are fundamental units in the kidney; hence, studying the glomeruli is pivotal for understanding renal function and pathology. Biological ...

Sichuan University

To enhance the drug delivery efficiency of hyaluronic acid (HA), we designed and prepared glycodendron and pyropheophorbide-a (Ppa)-functionalized HA (HA-Ppa-Dendron) as a nanosystem for cancer photodynamic therapy. Linear Ppa-modified HA (HA-Ppa) was also prepared as a control. Cellular uptake of both polymers by MDA-MB-231 cells led to mitochondrial dysfunction and generation of reactive oxygen species under the irradiation of a laser. Compared to the linear polymer, HA-Ppa-Dendron had higher molecular weight, a more compact nanoscale particle size, and a dendritic structure, resulting in a much longer blood circulation time and higher tumor accumulation. HA-Ppa-Dendron outperformed HA-Ppa in inhibiting cell growth, with 60 % of tumors was eradicated under laser irradiation. Tumor growth inhibition (TGI) up to 99.2 % was achieved from HA-Ppa-Dendron, which was much higher than that of HA-Ppa (50.6 %). Therefore, glycodendron-functionalized HAs by integration of HA and dendritic polymers may act as efficient anti-cancer nanomedicine.

Glycodendron/pyropheophorbide-a (Ppa)-functionalized hyaluronic acid as a nanosystem for tumor photodynamic therapy.

Vascular network labeling in transparent tissues provides more complete information on blood vessels. To achieve a fast and efficient method for vascular network labeling in transparent tissues, we compared various vascular labeling methods under different tissue clearing protocols. FITC-Dextran labeling and CUBIC cleaning treatment were found to be the best options for vascular network labeling in cleared mouse tissues. Satisfactory labeling of vascular networks in various organs can be achieved by selecting FITC-Dextran with different molecular weights and different administration methods.

Article	Year
Glycodendron/pyropheophorbide-a (Ppa)-functionalized hyaluronic acid as a nanosystem for tumor photodynamic therapy. Carbohydrate polymers\| PubMed ID: 32829865	2020
A simple and effective vascular network labeling method for transparent tissues of mice. Journal of biophotonics\| PubMed ID: 37017248	2023

Yaping Wu

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