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09:50 min
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February 09, 2024
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This study presents an easy-to-use, complete, and simple set of methods to label and analyze glomeruli from CUBIC-cleared mouse kidneys. Data such as glomerulus number and volume can be obtained easily and reliably using fluorescein isothiocyanate (FITC)-Dextran, light sheet fluorescence microscopy (LSFM), or common confocal microscopy and software such as Imaris.
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Cite this Article
Bai, L., Wu, Y., Dai, W., Shi, Q., Wu, L., Zhang, J., Zheng, L. An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli. J. Vis. Exp. (204), e65973, doi:10.3791/65973 (2024).
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