Postoperative Ileus Murine Model

Most patients experience postoperative ileus (POI) after surgery, which is associated with increased morbidity, mortality, and hospitalization time. POI ...

Capital Medical University

Diced electrophoresis gel (DEG) assay is a methodology to identify enzymes with a specified activity in complex cell or tissue lysates by means of two-dimensional separation using isoelectric focusing and native PAGE, followed by dicing of the gel into small pieces that are assayed separately, and digestion and peptide fingerprinting to identify the protein(s) of interest in positive wells. The existing hand-made system has some disadvantages, and here we describe the development and validation of an improved cutter-plate system that enables simple, reliable and reproducible DEG assay in a 384-well plate-based format with signal readout using fluorometric or LC-MS-based reaction monitoring. To illustrate the usefulness of this system, we describe its application to profile esterase activities in ovarian adenocarcinoma SKOV3 cell lysate and mouse liver lysate that activate a fluorogenic substrate, fluorescein dibutyrate (FDBu), as well as esterase activities in mouse liver lysate that activate S-bromobenzylglutathione dicyclopentyl ester (BBGDC), a prodrug of anti-tumor agent S-bromobenzylglutathione. The activity spot patterns detected for FDBu and BBGDC were completely different, indicating that different metabolic systems are involved in hydrolysis of these substrates. The major detected spot in each case was identified. The developed system provides a highly reproducible general assay platform that should be useful for characterizing novel protein functions in complex bio-samples, as well as enzymomics studies.

Development and validation of an improved diced electrophoresis gel assay cutter-plate system for enzymomics studies.

Controversy exists about the benefit of additional surgery after endoscopic submucosal dissection (ESD) for early gastric cancer (EGC).

Factors associated with overall survival in early gastric cancer patients who underwent additional surgery after endoscopic submucosal dissection.

Postoperative ileus (POI) is characterized by the activation of inflammation triggered by tissue damage. Damage-associated molecular patterns (DAMPs) reportedly induce local inflammation after injury. However, the impact of DAMPs on intestinal resident lymphocytes during POI remains poorly elucidated.

Article	Year
Development and validation of an improved diced electrophoresis gel assay cutter-plate system for enzymomics studies. Biochimica et biophysica acta. Proteins and proteomics\| PubMed ID: 29928991	2019
Factors associated with overall survival in early gastric cancer patients who underwent additional surgery after endoscopic submucosal dissection. World journal of clinical cases\| PubMed ID: 33869595	2021
Damage-induced NAD release activates intestinal CD4+ and CD8+ T cell via P2X7R signaling. Cellular immunology\| PubMed ID: 36746070	2023

Fandi Bu

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