Xin-Hui Xing

Department of Chemical Engineering, Tsinghua University

Affiliated withTsinghua University Shenzhen Bay laboratory Tsinghua Shenzhen International Graduate School

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JoVE Journal Publications

ArticleTotal : 2	Year
Publication title	2025
Publication title	2026

Other Publications

Article	Year
Rapid detection of a gfp-marked Enterobacter aerogenes under anaerobic conditions by aerobic fluorescence recovery. FEMS microbiology letters\| PubMed ID: 16006057	2005
A simplified method for assay of hydrogenase activities of H2 evolution and uptake in Enterobacter aerogenes. Biotechnology letters\| PubMed ID: 16132849	2005
Biological wastewater treatment by a bioreactor with repeated coupling of aerobes and anaerobes aiming at on-site reduction of excess sludge. Water science and technology : a journal of the International Association on Water Pollution Research\| PubMed ID: 16841729	2006
Functional expression of the particulate methane mono-oxygenase gene in recombinant Rhodococcus erythropolis. FEMS microbiology letters\| PubMed ID: 16978347	2006
[Study on the structure and function of a stable methane-oxidizing mixed microbial consortium]. Wei sheng wu xue bao = Acta microbiologica Sinica\| PubMed ID: 17436634	2007
Enhanced ozonation of simulated dyestuff wastewater by microbubbles. Chemosphere\| PubMed ID: 17467036	2007
Enhanced sludge solubilization by microbubble ozonation. Chemosphere\| PubMed ID: 18328535	2008
Enhanced coagulation of ferric chloride aided by tannic acid for phosphorus removal from wastewater. Chemosphere\| PubMed ID: 18395769	2008
Analysis of the mechanism of sludge ozonation by a combination of biological and chemical approaches. Water research\| PubMed ID: 18976790	2009
Optimization of effect factors for mycelial growth and exopolysaccharide production by Schizophyllum commune. Applied biochemistry and biotechnology\| PubMed ID: 19156366	2010
Paraffin oil as a "methane vector" for rapid and high cell density cultivation of Methylosinus trichosporium OB3b. Applied microbiology and biotechnology\| PubMed ID: 19219428	2009
Characteristics of hydrogen and methane production from cornstalks by an augmented two- or three-stage anaerobic fermentation process. Bioresource technology\| PubMed ID: 19231169	2009
Application of combined physicochemical and biological processes for enhanced treatment of avermectin fermentation wastewater. Water science and technology : a journal of the International Association on Water Pollution Research\| PubMed ID: 19237772	2009
Progress and perspectives of sludge ozonation as a powerful pretreatment method for minimization of excess sludge production. Water research\| PubMed ID: 19282018	2009
Temperature influence on fluorescence intensity and enzyme activity of the fusion protein of GFP and hyperthermophilic xylanase. Applied microbiology and biotechnology\| PubMed ID: 19390851	2009
Correcting for the inner filter effect in measurements of fluorescent proteins in high-cell-density cultures. Analytical biochemistry\| PubMed ID: 19394302	2009
Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine. FEMS microbiology ecology\| PubMed ID: 19515201	2009
Systematic analysis of biochemical performance and the microbial community of an activated sludge process using ozone-treated sludge for sludge reduction. Bioresource technology\| PubMed ID: 19515555	2009
Expression of NAD+-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H2 production. Biotechnology letters\| PubMed ID: 19533026	2009
Changes in biomass activity and characteristics of activated sludge exposed to low ozone dose. Chemosphere\| PubMed ID: 19698970	2009
Alteration of hydrogen metabolism of ldh-deleted Enterobacter aerogenes by overexpression of NAD+-dependent formate dehydrogenase. Applied microbiology and biotechnology\| PubMed ID: 19830418	2010
Reconstruction of the violacein biosynthetic pathway from Duganella sp. B2 in different heterologous hosts. Applied microbiology and biotechnology\| PubMed ID: 20012278	2010
Biochemical analysis and kinetic modeling of the thermal inactivation of MBP-fused heparinase I: implications for a comprehensive thermostabilization strategy. Biotechnology and bioengineering\| PubMed ID: 21445884	2011
Bioengineering of the Enterobacter aerogenes strain for biohydrogen production. Bioresource technology\| PubMed ID: 21764301	2011
Luciferase and fluorescent protein as dual reporters analyzing the effect of n-dodecyltrimethylammonium bromide on the physiology of Pseudomonas putida. Applied microbiology and biotechnology\| PubMed ID: 22075632	2012
Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase. Enzyme and microbial technology\| PubMed ID: 22112777	2011
Improved hydrogen production under microaerophilic conditions by overexpression of polyphosphate kinase in Enterobacter aerogenes. Enzyme and microbial technology\| PubMed ID: 22112830	2011
Combination of site-directed mutagenesis and calcium ion addition for enhanced production of thermostable MBP-fused heparinase I in recombinant Escherichia coli. Applied microbiology and biotechnology\| PubMed ID: 22588503	2012
Improvement of hydrogen productivity by introduction of NADH regeneration pathway in Clostridium paraputrificum. Applied biochemistry and biotechnology\| PubMed ID: 22592776	2012
Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection. Journal of biotechnology\| PubMed ID: 23073152	2013
Thermostable alcohol dehydrogenase from Thermococcus kodakarensis KOD1 for enantioselective bioconversion of aromatic secondary alcohols. Applied and environmental microbiology\| PubMed ID: 23354700	2013
Active inclusion bodies of acid phosphatase PhoC: aggregation induced by GFP fusion and activities modulated by linker flexibility. Microbial cell factories\| PubMed ID: 23497261	2013
Comprehensive phylogenetic diversity of [FeFe]-hydrogenase genes in termite gut microbiota. Microbes and environments\| PubMed ID: 24240187	2013
Controllable production of low molecular weight heparins by combinations of heparinase I/II/III. Carbohydrate polymers\| PubMed ID: 24299802	2014
Atmospheric and room temperature plasma (ARTP) as a new powerful mutagenesis tool. Applied microbiology and biotechnology\| PubMed ID: 24769904	2014
Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community. Bioresource technology\| PubMed ID: 25459816	2015
High crude violacein production from glucose by Escherichia coli engineered with interactive control of tryptophan pathway and violacein biosynthetic pathway. Microbial cell factories\| PubMed ID: 25592762	2015
Performance and microbial community of carbon nanotube fixed-bed microbial fuel cell continuously fed with hydrothermal liquefied cornstalk biomass. Bioresource technology\| PubMed ID: 25780905	2015
Culture characteristics of the atmospheric and room temperature plasma-mutated Spirulina platensis mutants in CO2 aeration culture system for biomass production. Journal of bioscience and bioengineering\| PubMed ID: 25795571	2015
DomSign: a top-down annotation pipeline to enlarge enzyme space in the protein universe. BMC bioinformatics\| PubMed ID: 25888481	2015
Quantitative evaluation of DNA damage and mutation rate by atmospheric and room-temperature plasma (ARTP) and conventional mutagenesis. Applied microbiology and biotechnology\| PubMed ID: 26025015	2015
Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes. Bioresource technology\| PubMed ID: 26188552	2015
Construction of a linker library with widely controllable flexibility for fusion protein design. Applied microbiology and biotechnology\| PubMed ID: 26394862	2016
Insights into the global regulation of anaerobic metabolism for improved biohydrogen production. Bioresource technology\| PubMed ID: 26476162	2016
Intermediate-sensor assisted push-pull strategy and its application in heterologous deoxyviolacein production in Escherichia coli. Metabolic engineering\| PubMed ID: 26506462	2016
Bioconversion of methanol to value-added mevalonate by engineered Methylobacterium extorquens AM1 containing an optimized mevalonate pathway. Applied microbiology and biotechnology\| PubMed ID: 26521242	2016
Characterization of a novel Acinetobacter baumannii xanthine dehydrogenase expressed in Escherichia coli. Biotechnology letters\| PubMed ID: 26543035	2016
A study on the effects of linker flexibility on acid phosphatase PhoC-GFP fusion protein using a novel linker library. Enzyme and microbial technology\| PubMed ID: 26777244	2016
Establishment of chondroitin B lyase-based analytical methods for sensitive and quantitative detection of dermatan sulfate in heparin. Carbohydrate polymers\| PubMed ID: 27083825	2016
Enhanced biohydrogen production from corn stover by the combination of Clostridium cellulolyticum and hydrogen fermentation bacteria. Journal of bioscience and bioengineering\| PubMed ID: 27150511	2016
Enrichment and characteristics of mixed methane-oxidizing bacteria from a Chinese coal mine. Applied microbiology and biotechnology\| PubMed ID: 27474022	2016
Xanthine dehydrogenase: An old enzyme with new knowledge and prospects. Bioengineered\| PubMed ID: 27537049	2016
Improved production of trans-4-hydroxy-l-proline by chromosomal integration of the Vitreoscilla hemoglobin gene into recombinant Escherichia coli with expression of proline-4-hydroxylase. Journal of bioscience and bioengineering\| PubMed ID: 27567047	2017
Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors. Biotechnology for biofuels\| PubMed ID: 27895708	2016
Biosensor-assisted transcriptional regulator engineering for Methylobacterium extorquens AM1 to improve mevalonate synthesis by increasing the acetyl-CoA supply. Metabolic engineering\| PubMed ID: 27919791	2017
Non-anticoagulant effects of low molecular weight heparins in inflammatory disorders: A review. Carbohydrate polymers\| PubMed ID: 28115102	2017
Erratum to: Continuous production of biohythane from hydrothermal liquefied cornstalk biomass via two-stage high-rate anaerobic reactors. Biotechnology for biofuels\| PubMed ID: 28174602	2017
Metabolic engineering of Escherichia coli cell factory for highly active xanthine dehydrogenase production. Bioresource technology\| PubMed ID: 28610971	2017
Discovery of enzymatically depolymerized heparins capable of treating Bleomycin-induced pulmonary injury and fibrosis in mice. Carbohydrate polymers\| PubMed ID: 28821136	2017
Maltose Utilization as a Novel Selection Strategy for Continuous Evolution of Microbes with Enhanced Metabolite Production. ACS synthetic biology\| PubMed ID: 28841296	2017
Effects of Enzymatically Depolymerized Low Molecular Weight Heparins on CCl-Induced Liver Fibrosis. Frontiers in pharmacology\| PubMed ID: 28871223	2017
Bioprocess engineering for biohythane production from low-grade waste biomass: technical challenges towards scale up. Current opinion in biotechnology\| PubMed ID: 28892667	2018
A rapid and specific colorimetric method for free tryptophan quantification. Talanta\| PubMed ID: 28917797	2018
Targeted mutagenesis: A sniper-like diversity generator in microbial engineering. Synthetic and systems biotechnology\| PubMed ID: 29062964	2017
Cre/loxP-Mediated Multicopy Integration of the Mevalonate Operon into the Genome of Methylobacterium extorquens AM1. Applied biochemistry and biotechnology\| PubMed ID: 29243041	2018
Design of Fusion Proteins for Efficient and Soluble Production of Immunogenic Ebola Virus Glycoprotein in Escherichia coli. Biotechnology journal\| PubMed ID: 29500882	2018
MiYA, an efficient machine-learning workflow in conjunction with the YeastFab assembly strategy for combinatorial optimization of heterologous metabolic pathways in Saccharomyces cerevisiae. Metabolic engineering\| PubMed ID: 29627507	2018
Breeding of Methanol-Tolerant Methylobacterium extorquens AM1 by Atmospheric and Room Temperature Plasma Mutagenesis Combined With Adaptive Laboratory Evolution. Biotechnology journal\| PubMed ID: 29729127	2018
Enhanced Production of Crude Violacein from Glucose in Escherichia coli by Overexpression of Rate-Limiting Key Enzyme(S) Involved in Violacein Biosynthesis. Applied biochemistry and biotechnology\| PubMed ID: 29797295	2018
Massilia violaceinigra sp. nov., a novel purple-pigmented bacterium isolated from glacier permafrost. International journal of systematic and evolutionary microbiology\| PubMed ID: 29851375	2018
Structural characterization and in vitro antioxidant activities of chondroitin sulfate purified from Andrias davidianus cartilage. Carbohydrate polymers\| PubMed ID: 29891311	2018
Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Nature communications\| PubMed ID: 29946130	2018
Improved sgRNA design in bacteria via genome-wide activity profiling. Nucleic acids research\| PubMed ID: 29982721	2018
Genome-wide screening identifies promiscuous phosphatases impairing terpenoid biosynthesis in Escherichia coli. Applied microbiology and biotechnology\| PubMed ID: 30229323	2018
Novel breeding approach for Japanese flounder using atmosphere and room temperature plasma mutagenesis tool. BMC genomics\| PubMed ID: 31035925	2019
Dynamics of transcription-translation coordination tune bacterial indole signaling. Nature chemical biology\| PubMed ID: 31873224	2020
Prokaryotic communities in multidimensional bottom-pit-mud from old and young pits used for the production of Chinese Strong-Flavor Baijiu. Food chemistry\| PubMed ID: 31901820	2020
Highly Efficient Capture of Marine Microbial Strains in Seawater Using Bare FeO Magnetic Beads. Current microbiology\| PubMed ID: 32103330	2020
Microbial microdroplet culture system (MMC): An integrated platform for automated, high-throughput microbial cultivation and adaptive evolution. Biotechnology and bioengineering\| PubMed ID: 32159223	2020
Establishment of CRISPR interference in Methylorubrum extorquens and application of rapidly mining a new phytoene desaturase involved in carotenoid biosynthesis. Applied microbiology and biotechnology\| PubMed ID: 32215707	2020
Hybrid nanoparticles with CO-responsive shells and fluorescence-labelled magnetic cores. Journal of materials chemistry. B\| PubMed ID: 32261388	2014
Electrochemically-responsive magnetic nanoparticles for reversible protein adsorption. Journal of materials chemistry. B\| PubMed ID: 32263099	2016
Increased stability and intracellular antioxidant activity of chlorogenic acid depend on its molecular interaction with wheat gluten hydrolysate. Food chemistry\| PubMed ID: 32387948	2020
Empowering a Methanol-Dependent via Adaptive Evolution Using a High-Throughput Microbial Microdroplet Culture System. Frontiers in bioengineering and biotechnology\| PubMed ID: 32733857	2020
Insights into the molecular-level effects of atmospheric and room-temperature plasma on mononucleotides and single-stranded homo- and hetero-oligonucleotides. Scientific reports\| PubMed ID: 32868795	2020
A heparin derivatives library constructed by chemical modification and enzymatic depolymerization for exploitation of non-anticoagulant functions. Carbohydrate polymers\| PubMed ID: 32933671	2020
Design and construction of chimeric linker library with controllable flexibilities for precision protein engineering. Methods in enzymology\| PubMed ID: 33482990	2021
Rewiring the native methanol assimilation metabolism by incorporating the heterologous ribulose monophosphate cycle into Methylorubrum extorquens. Metabolic engineering\| PubMed ID: 33493644	2021
Guide-target mismatch effects on dCas9-sgRNA binding activity in living bacterial cells. Nucleic acids research\| PubMed ID: 33503261	2021
Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1. Biotechnology journal\| PubMed ID: 33595188	2021
Effects of bioactive components of Pu-erh tea on gut microbiomes and health: A review. Food chemistry\| PubMed ID: 33743430	2021
[Technology development and instrumentation of a high-throughput and automated microbial microdroplet culture system for microbial evolution and screening]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology\| PubMed ID: 33783163	2021
Genome-wide genotype-phenotype associations in microbes. Journal of bioscience and bioengineering\| PubMed ID: 33895083	2021
Advanced strategies and tools to facilitate and streamline microbial adaptive laboratory evolution. Trends in biotechnology\| PubMed ID: 33958227	2022
[Functional discovery and production technology for natural bioactive peptides]. Sheng wu gong cheng xue bao = Chinese journal of biotechnology\| PubMed ID: 34227301	2021
Construction and characterization of novel bifunctional fusion proteins composed of alcohol dehydrogenase and NADH oxidase with efficient oxidized cofactor regeneration. Biotechnology and applied biochemistry\| PubMed ID: 34269481	2021
Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure. Glycoconjugate journal\| PubMed ID: 34515908	2021
New Method for Genome-Scale Functional Genomic Study in Bacteria with Superior Performance: CRISPR Interference Screen. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 34709614	2022
A versatile toolbox for CRISPR-based genome engineering in Pichia pastoris. Applied microbiology and biotechnology\| PubMed ID: 34773154	2021
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast. ACS synthetic biology\| PubMed ID: 35089702	2022
Turning C1-gases to isobutanol towards great environmental and economic sustainability via innovative biological routes: two birds with one stone. Biotechnology for biofuels and bioproducts\| PubMed ID: 36221148	2022
Sodium formate redirects carbon flux and enhances heterologous mevalonate production in Methylobacterium extorquens AM1. Biotechnology journal\| PubMed ID: 36424513	2023
Cross-species binding spectrum of ACE2 to SARS-CoV-2 RBD. Science China. Life sciences\| PubMed ID: 36459354	2023
Single-cell microliter-droplet screening system (MISS Cell): An integrated platform for automated high-throughput microbial monoclonal cultivation and picking. Biotechnology and bioengineering\| PubMed ID: 36477904	2023
CRISPRi-microfluidics screening enables genome-scale target identification for high-titer protein production and secretion. Metabolic engineering\| PubMed ID: 36572334	2023
Recent advances of integrated microfluidic suspension cell culture system. Engineering biology\| PubMed ID: 36970555	2021
Mining and Validation of Novel Hemp Seed-Derived DPP-IV-Inhibiting Peptides Using a Combination of Multi-omics and Molecular Docking. Journal of agricultural and food chemistry\| PubMed ID: 37058363	2023
Exploration of DPP-IV Inhibitory Peptide Design Rules Assisted by the Deep Learning Pipeline That Identifies the Restriction Enzyme Cutting Site. ACS omega\| PubMed ID: 37901493	2023
Deep-learning-assisted Sort-Seq enables high-throughput profiling of gene expression characteristics with high precision. Science advances\| PubMed ID: 37939173	2023
Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes. Science China. Life sciences\| PubMed ID: 38273187	2024
Identification of the mutual gliding locus as a factor for gut colonization in non-native bee hosts using the ARTP mutagenesis. Microbiome\| PubMed ID: 38778376	2024
Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting . ACS synthetic biology\| PubMed ID: 38825826	2024
Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth. Nature communications\| PubMed ID: 39013920	2024
"Two-birds-one-stone" oral nanotherapeutic designed to target intestinal integrins and regulate redox homeostasis for UC treatment. Science advances\| PubMed ID: 39047093	2024
Characterization of maltose-binding protein-fused heparinases with enhanced thermostability by application of rigid and flexible linkers. Biotechnology and applied biochemistry\| PubMed ID: 39072851	2025
GLiDe: a web-based genome-scale CRISPRi sgRNA design tool for prokaryotes. BMC bioinformatics\| PubMed ID: 39754035	2025