High-Throughput Screening of L-Lactic Acid-Hyperproducing Bacillus coagulans Mutants Using an Automated Droplet Microfluidic Platform

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11:28 min

July 28th, 2026

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Xin Da^*¹^,² , Shuang Li^*² , Qingyuan Wang³ , Xunxun Luo² , Dezhi Geng⁴ , Xiaojie Guo⁵ , Liyan Wang⁵ , Dongliang Jiang³ , Yi Wang²^,⁶^,⁷ , Xin-Hui Xing²^,⁸^,⁹^,¹⁰ , Zhenglian Xue¹ , Chong Zhang²^,⁶^,⁷^,¹⁰

¹College of Biology and Food Engineering, Anhui Polytechnic University, ²Ministry of Education Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, ³Henan Jindan Lactic Acid Technology Co., Ltd., ⁴Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, ⁵Jiangsu TMAXTREE Biotechnology Co., Ltd., ⁶Beijing Key Laboratory of Recombinant Proteins and Biomanufacturing, ⁷State Key Laboratory of Green Biomanufacturing, ⁸Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, ⁹Key Laboratory of Active Proteins and Peptides, Green Biomanufacturing of Guangdong Higher Education Institutes, Tsinghua Shenzhen International Graduate School, ¹⁰Center for Synthetic and Systems Biology, Tsinghua University

^* These authors contributed equally

This protocol presents a standard high-throughput screening workflow for industrial microorganisms. It involves constructing a mutant library via ARTP mutagenesis, performing positive droplet screening with automated microfluidics, and subsequently verifying the results. The workflow’s reliability was validated by screening high-yield B. coagulans strains.