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Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow

Tuberculosis is a deadly disease, and the emergence of antibiotic drug resistance in the causative agent bacterium, Mycobacterium tuberculosis, worsens ...

University of California, San Francisco

Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens. Despite this importance, few tools are available for designing multiplex primers.

Jason D. Limberis

Biography

JoVE Journal Publications

Other Publications