iterative-bleaching-extends-multiplicity-with-use-staining-automation

Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

Iterative Bleaching Extends Multiplicity (IBEX) is a highly sensitive tyramide signal amplification (TSA)-based multi-round fluorescence imaging technique ...

Cleveland Clinic Foundation

Although virus-host interactions are usually studied in a single cell type using in vitro assays in immortalized cell lines or isolated cell populations, it is important to remember that what is happening inside one infected cell does not translate to understanding how an infected cell behaves in a tissue, organ or whole organism. Infections occur in complex tissue environments, which contain a host of factors that can alter the course of the infection, including immune cells, non-immune cells and extracellular-matrix components. These factors affect how the host responds to the virus and form the basis of the protective response. To understand virus infection, tools are needed that can profile the tissue environment. This Review highlights methods to study virus-host interactions in the infection microenvironment.

Understanding virus-host interactions in tissues.

Transcriptional analysis is a powerful tool to help decipher the host response to filovirus infections. In this chapter, we describe how transcriptional analysis can be performed on samples obtained from nonhuman primates infected with filoviruses, specifically Marburg virus, for assessment of the RNA changes over the course of the infection and disease.

Article	Year
Understanding virus-host interactions in tissues. Nature microbiology\| PubMed ID: 37488255	2023
Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 39585635	2025
Finding the lock to fit the key: Ebola virus entry. Nature reviews. Microbiology\| PubMed ID: 40707621	2025

Emily Speranza

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