Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

Anna E. Tseng; Aoife O'Connell; Aaron Benhamou; Shivraj Yabaji; Igor Kramnik; Nicholas A. Crossland; Emily Speranza

doi:10.3791/67853

Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Iterative Bleaching Extends Multiplicity with Use of Staining Automation for Core Facilities

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04:52 min

August 6, 2025

DOI: 10.3791/67853-v

Anna E. Tseng^1,2, Aoife O'Connell¹, Aaron Benhamou^1,3, Shivraj Yabaji^1,2, Igor Kramnik^1,2, Nicholas A. Crossland^1,2,3, Emily Speranza⁴

¹National Emerging Infectious Diseases Laboratories (NEIDL),Boston University, ²Department of Pathology and Laboratory Medicine,Boston University Chobanian and Avedisian School of Medicine, ³Department of Virology, Immunology and Microbiology,Boston University Chobanian and Avedisian School of Medicine, ⁴Florida Research and Innovation Center,Cleveland Clinic Foundation

Here, we describe the application of the Iterative Bleaching Extends Multiplicity (IBEX) method utilizing commercially available, automated tissue stainers and optimization considerations for the use of tyramide signal amplification-based reagents.

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