an-intelligent-structural-color-hydrogel-patch-integrating-diagnosis

An Intelligent Structural Color Hydrogel Patch Integrating Diagnosis and Treatment for the Management of Chronic Osteomyelitis

Chronic osteomyelitis (COM) is a persistent bone infection that is difficult to treat with conventional antibiotics due to issues such as drug resistance ...

Tongde Hospital Of Zhejiang Province

Objective To prepare biomimetic bone material by reconstructing type Ⅰ collagen combined with polyaspartic acid. Methods By acid hydrolysis,rat tail type Ⅰ collagen was decomposed into collagen fibers,which were then placed in the calcium phosphate mineralization solution. Under the cross-linking of glutaraldehyde,the collagen fibers were reconstructed and assembled into collagen fibers,and the calcium phosphate crystals were wrapped in the inner side of the collagen fibers for biomineralizationin. After poly aspartate acid was added,calcium hydroxyapatite calcium precursor was added into the collagen fibers to simulate thebiomimetic biomineralizationin the human body. After mineralization for 3-9 days,the bone mineralization process was observed by transmission electron microscopy and electron diffraction. Results Transmission electron microscopy and electron diffraction displayed that,after 3 days of mineralization,calcium hydroxyapatite precursor was wrapped in the collagen fiber gap,and the collagen fiber was partially mineralized. After 9 days of mineralization,calcium hydroxyapatite precursor completely infiltrated into the collagen fiber,and the amorphous calcium phosphate was transformed into hydroxyapatite calcium crystal. Thus,the simulation of bone mineralization was completed,and collagen type Ⅰ collagen/hydroxyapatite calcium biomimetic bone material was formed. Conclusion Reconstruction of type Ⅰ collagen combined with polyaspartic acid can prepare biomimetic bone material that has close chemical composition and molecular structure to the human bone tissue.

Application of Recombinant Collagen Type Ⅰ Combined with Polyaspartic Acid in Biomimetic Biomineralization.

To introduce and report the preliminary results of 7 old patients with fractures and dislocations of the elbow.

[Surgical treatment for the fractures and dislocations of the elbow in old patients].

To evaluate the middle-long-term clinical effects of unilateral and bilateral percutaneous kyphoplasty(PKP) for vertebral fragility fracture in the elderly.

[Mid and long-term clinical effects of percutaneous kyphoplasty with two methods for vertebral fragility fracture in the elderly].

To compare clinical effects of artificial bone and autogenous bone in internal fixation of complex calcaneal fracture with profitated plate.

[Case control study on the application of artificial bone and autogenous bone in internal fixation of complex calcaneal fracture with special-shaped plate].

Osteomyelitis is an acute or chronic inflammatory bone disease with a high disability rate. As an anti-inflammatory factor, peroxisome proliferator activated receptor-γ (PPAR-γ) is not only implicated in a variety of inflammatory responses but also regulates osteoblast differentiation and bone mass. However, the role of PPAR-γ in osteomyelitis is not fully understood. In the present study, we demonstrated that PPAR-γ showed a lower expression level in infected bone tissue of osteomyelitis patients as compared with uninfected bone tissue from nonosteomyelitis patients with fracture of the hip. We applied lipopolysaccharides (LPSs) in MC3T3-E1 osteoblast precursor cell line as an in vitro model for osteomyelitis. LPS treatment increased osteomyelitis-associated inflammatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), whereas PPAR-γ levels and cell viability in MC3T3-E1 cells were suppressed. PPAR-γ antagonist GW9662 further enhanced IL-6 and TNF-α levels, and decreased cell viability in the presence of LPS treatment. In contrast, PPAR-γ agonist pioglitazone antagonized the effect of LPS treatment in MC3T3-E1 cells. These findings suggest that PPAR-γ downregulation is associated with the inflammation and progression of osteomyelitis, and PPAR-γ agonist could serve as a therapeutic strategy to attenuate inflammatory responses. This study provides novel insights into the physiopathogenesis of osteomyelitis and future study is required to validate the findings in animal model and uncover the molecular mechanism of PPAR-γ-dependent anti-inflammation in osteoblasts.

PPAR-γ agonist pioglitazone alleviates inflammatory response induced by lipopolysaccharides in osteoblast cells.

To predict the malignancy of 1-5 cm gastric gastrointestinal stromal tumors (GISTs) by machine learning (ML) on CT images using three models - Logistic Regression (LR), Decision Tree (DT) and Gradient Boosting Decision Tree (GBDT).

Article	Year
Application of Recombinant Collagen Type Ⅰ Combined with Polyaspartic Acid in Biomimetic Biomineralization. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae\| PubMed ID: 28695800	2017
[Surgical treatment for the fractures and dislocations of the elbow in old patients]. Zhongguo gu shang = China journal of orthopaedics and traumatology\| PubMed ID: 29327556	2017
[Mid and long-term clinical effects of percutaneous kyphoplasty with two methods for vertebral fragility fracture in the elderly]. Zhongguo gu shang = China journal of orthopaedics and traumatology\| PubMed ID: 29417773	2017
[Case control study on the application of artificial bone and autogenous bone in internal fixation of complex calcaneal fracture with special-shaped plate]. Zhongguo gu shang = China journal of orthopaedics and traumatology\| PubMed ID: 34569203	2021
PPAR-γ agonist pioglitazone alleviates inflammatory response induced by lipopolysaccharides in osteoblast cells. Journal of orthopaedic research : official publication of the Orthopaedic Research Society\| PubMed ID: 35072290	2022
Machine learning for predicting the risk stratification of 1-5 cm gastric gastrointestinal stromal tumors based on CT. BMC medical imaging\| PubMed ID: 37415125	2023

Huajun Yu

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