measurement-pulmonary-artery-pressure-rats-using-right-heart

Measurement of Pulmonary Artery Pressure in Rats Using Right Heart Catheterization

Pulmonary artery pressure (PAP) measured by right heart catheterization is considered the gold standard for diagnosing pulmonary hypertension. Rats have ...

augmented-reality-tourism-technology-based-on-an-improved-orb

Augmented Reality Tourism Technology Based on an Improved ORB Algorithm and Homography Matrix

This study proposes an improved feature-point matching and 3D registration method for augmented reality tourism applications. This method addresses issues ...

Key Laboratory of Extreme Environmental Medicine of the Ministry of Education

Army Medical University

Business School, Xianning Polytechnic

Abnormal activation of the IGF1R signaling pathway accelerates melanoma development and metastases. RNAi systems with complex cloning procedures and unsatisfactory efficiency in suppressing gene expression have become the technical difficulties that hinder their utility when studying gene knockdown. Here we established a simplified adenovirus-mediated gene knockdown system by which a single adenoviral vector carries multiple siRNA fragments that can effectively suppress IGF1R expression in melanoma cells. We first generated the adenovirus that simultaneously expresses three human or mouse siRNAs targeting IGF1R (AdRIGF1R-OK). qRT-PCR and immunofluorescence staining revealed that IGF1R expression was significantly decreased in the melanoma cells that were infected with AdRIGF1R-OK. Bioluminescence imaging showed that the size of the tumor formed by the xenografts infected with AdRIGF1R-OK was significantly smaller than that of the controls. Annexin V-FITC flow cytometry assay, immunofluorescence staining for cleaved caspase-3, and Hoechst staining showed that more cells underwent apoptosis after infection with AdRIGF1R-OK. Luciferase reporter assay, crystal violet cell viability assay, and cell-cycle analysis showed that the proliferation of melanoma cells infected with AdRIGF1R-OK was significantly decreased compared to the controls. This study demonstrates that the OK system is effective in silencing gene expression, with promising potential to treat melanoma and other diseases.

ArticleTotal : 2	Year
Publication title	2025
Publication title	2026

Fang Deng

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