Brian T. Chait

Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University

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JoVE Journal Publications

ArticleTotal : 1	Year
Publication title Cited by 31	2007

Other Publications

Article	Year
SYT associates with human SNF/SWI complexes and the C-terminal region of its fusion partner SSX1 targets histones. The Journal of biological chemistry\| PubMed ID: 11734557	2002
N-terminal contributions of the gamma-subunit of fetal hemoglobin to its tetramer strength: remote effects at subunit contacts. Protein science : a publication of the Protein Society\| PubMed ID: 11742119	2002
X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity. Nature\| PubMed ID: 11796999	2002
Mass spectrometric analysis of the N terminus of translational initiation factor eIF4G-1 reveals novel isoforms. The Journal of biological chemistry\| PubMed ID: 11821405	2002
On the nature of the chemical noise in MALDI mass spectra. Journal of the American Society for Mass Spectrometry\| PubMed ID: 11838016	2002
The N-CoR-HDAC3 nuclear receptor corepressor complex inhibits the JNK pathway through the integral subunit GPS2. Molecular cell\| PubMed ID: 11931768	2002
Requirement of Ca2+ and CaMKII for Stat1 Ser-727 phosphorylation in response to IFN-gamma. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 11972023	2002
Crystal structure and mechanism of a calcium-gated potassium channel. Nature\| PubMed ID: 12037559	2002
The open pore conformation of potassium channels. Nature\| PubMed ID: 12037560	2002
Tyrosine sulfation of CCR5 N-terminal peptide by tyrosylprotein sulfotransferases 1 and 2 follows a discrete pattern and temporal sequence. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 12169668	2002
Proteomic analysis of the mammalian nuclear pore complex. The Journal of cell biology\| PubMed ID: 12196509	2002
ABI5 acts downstream of ABI3 to execute an ABA-dependent growth arrest during germination. The Plant journal : for cell and molecular biology\| PubMed ID: 12410810	2002
Ezh2 controls B cell development through histone H3 methylation and Igh rearrangement. Nature immunology\| PubMed ID: 12496962	2003
CIAP1 and the serine protease HTRA2 are involved in a novel p53-dependent apoptosis pathway in mammals. Genes & development\| PubMed ID: 12569127	2003
Detection of secreted peptides by using hypothesis-driven multistage mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 12591958	2003
A modular cross-linking approach for exploring protein interactions. Journal of the American Chemical Society\| PubMed ID: 12603129	2003
X-ray structure of a voltage-dependent K+ channel. Nature\| PubMed ID: 12721618	2003
Localization of the PE methylation pathway and SR-BI to the canalicular membrane: evidence for apical PC biosynthesis that may promote biliary excretion of phospholipid and cholesterol. Journal of lipid research\| PubMed ID: 12810817	2003
Dynorphin A(1-17) biotransformation in striatum of freely moving rats using microdialysis and matrix-assisted laser desorption/ionization mass spectrometry. Journal of neurochemistry\| PubMed ID: 12887680	2003
"De novo" peptide sequencing by MALDI-quadrupole-ion trap mass spectrometry: a preliminary study. Journal of the American Society for Mass Spectrometry\| PubMed ID: 12954169	2003
Genetic and biochemical evaluation of the importance of Cdc6 in regulating mitotic exit. Molecular biology of the cell\| PubMed ID: 12960422	2003
Structure of microcin J25, a peptide inhibitor of bacterial RNA polymerase, is a lassoed tail. Journal of the American Chemical Society\| PubMed ID: 14531691	2003
Biophysical methods: doing more with less. Current opinion in structural biology\| PubMed ID: 14568606	2003
Virtual gating and nuclear transport: the hole picture. Trends in cell biology\| PubMed ID: 14624840	2003
Improved beta-elimination-based affinity purification strategy for enrichment of phosphopeptides. Analytical chemistry\| PubMed ID: 14670042	2003
Targeted proteomic study of the cyclin-Cdk module. Molecular cell\| PubMed ID: 15200949	2004
POT1-interacting protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex. Genes & development\| PubMed ID: 15231715	2004
Analysis of protein phosphorylation by hypothesis-driven multiple-stage mass spectrometry. Analytical chemistry\| PubMed ID: 15283590	2004
TIN2 binds TRF1 and TRF2 simultaneously and stabilizes the TRF2 complex on telomeres. The Journal of biological chemistry\| PubMed ID: 15316005	2004
E protein silencing by the leukemogenic AML1-ETO fusion protein. Science (New York, N.Y.)\| PubMed ID: 15333839	2004
Dual functions, clamp opening and primer-template recognition, define a key clamp loader subunit. Journal of molecular biology\| PubMed ID: 15364574	2004
Characterization of karyopherin cargoes reveals unique mechanisms of Kap121p-mediated nuclear import. Molecular and cellular biology\| PubMed ID: 15367670	2004
Domain-specific incorporation of noninvasive optical probes into recombinant proteins. Journal of the American Chemical Society\| PubMed ID: 15506763	2004
Components of coated vesicles and nuclear pore complexes share a common molecular architecture. PLoS biology\| PubMed ID: 15523559	2004
Preferential phosphorylation of R-domain Serine 768 dampens activation of CFTR channels by PKA. The Journal of general physiology\| PubMed ID: 15657296	2005
Channel function is dissociated from the intrinsic kinase activity and autophosphorylation of TRPM7/ChaK1. The Journal of biological chemistry\| PubMed ID: 15781465	2005
Proteomic and genomic characterization of chromatin complexes at a boundary. The Journal of cell biology\| PubMed ID: 15824130	2005
N-terminal acetylation and protonation of individual hemoglobin subunits: position-dependent effects on tetramer strength and cooperativity. Protein science : a publication of the Protein Society\| PubMed ID: 15929996	2005
Polyglutamine-expanded ataxin-7 inhibits STAGA histone acetyltransferase activity to produce retinal degeneration. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 15932940	2005
Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF. Cell\| PubMed ID: 15960975	2005
MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. Molecular cell\| PubMed ID: 15989967	2005
The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly. The Journal of cell biology\| PubMed ID: 16027220	2005
Long range allosteric control of cytoplasmic dynein ATPase activity by the stalk and C-terminal domains. The Journal of biological chemistry\| PubMed ID: 16030013	2005
Fluorescent proteins as proteomic probes. Molecular & cellular proteomics : MCP\| PubMed ID: 16155292	2005
I-DIRT, a general method for distinguishing between specific and nonspecific protein interactions. Journal of proteome research\| PubMed ID: 16212429	2005
A method for the rapid and efficient elution of native affinity-purified protein A tagged complexes. Journal of proteome research\| PubMed ID: 16335973	2005
Quantitative analysis of protein phosphorylation in mouse brain by hypothesis-driven multistage mass spectrometry. Analytical chemistry\| PubMed ID: 16351129	2005
The mobile nucleoporin Nup2p and chromatin-bound Prp20p function in endogenous NPC-mediated transcriptional control. The Journal of cell biology\| PubMed ID: 16365162	2005
Linker chains of the gigantic hemoglobin of the earthworm Lumbricus terrestris: primary structures of linkers L2, L3, and L4 and analysis of the connectivity of the disulfide bonds in linker... Proteins\| PubMed ID: 16425180	2006
Simple fold composition and modular architecture of the nuclear pore complex. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 16461911	2006
Protease accessibility laddering: a proteomic tool for probing protein structure. Structure (London, England : 1993)\| PubMed ID: 16615907	2006
Synergistic functions of SII and p300 in productive activator-dependent transcription of chromatin templates. Cell\| PubMed ID: 16630816	2006
Regulation of hypermutation by activation-induced cytidine deaminase phosphorylation. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 16723391	2006
A PHD finger of NURF couples histone H3 lysine 4 trimethylation with chromatin remodelling. Nature\| PubMed ID: 16728976	2006
Induction of autophagy in axonal dystrophy and degeneration. The Journal of neuroscience : the official journal of the Society for Neuroscience\| PubMed ID: 16885219	2006
Tracking and elucidating alphavirus-host protein interactions. The Journal of biological chemistry\| PubMed ID: 16895903	2006
Chemistry. Mass spectrometry: bottom-up or top-down? Science (New York, N.Y.)\| PubMed ID: 17023639	2006
Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo. The Journal of cell biology\| PubMed ID: 17116750	2006
Yng1 PHD finger binding to H3 trimethylated at K4 promotes NuA3 HAT activity at K14 of H3 and transcription at a subset of targeted ORFs. Molecular cell\| PubMed ID: 17157260	2006
Chromosomal enrichment and activation of the aurora B pathway are coupled to spatially regulate spindle assembly. Developmental cell\| PubMed ID: 17199039	2007
Long-distance combinatorial linkage between methylation and acetylation on histone H3 N termini. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 17284592	2007
Cell-cycle-dependent phosphorylation of the nuclear pore Nup107-160 subcomplex. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 17360435	2007
Efficiency, selectivity, and robustness of nucleocytoplasmic transport. PLoS computational biology\| PubMed ID: 17630825	2007
Human embryonic, fetal, and adult hemoglobins have different subunit interface strengths. Correlation with lifespan in the red cell. Protein science : a publication of the Protein Society\| PubMed ID: 17656582	2007
Prediction of cyclin-dependent kinase phosphorylation substrates. PloS one\| PubMed ID: 17668044	2007
Crystal structure of a Kir3.1-prokaryotic Kir channel chimera. The EMBO journal\| PubMed ID: 17703190	2007
Methylation of a histone mimic within the histone methyltransferase G9a regulates protein complex assembly. Molecular cell\| PubMed ID: 17707231	2007
Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 17726112	2007
Comprehensive analysis of diverse ribonucleoprotein complexes. Nature methods\| PubMed ID: 17922018	2007
Multifunctional class I transcription in Trypanosoma brucei depends on a novel protein complex. The EMBO journal\| PubMed ID: 17972917	2007
Determining the architectures of macromolecular assemblies. Nature\| PubMed ID: 18046405	2007
The molecular architecture of the nuclear pore complex. Nature\| PubMed ID: 18046406	2007
Affinity isolation and I-DIRT mass spectrometric analysis of the Escherichia coli O157:H7 Sakai RNA polymerase complex. Journal of bacteriology\| PubMed ID: 18083804	2008
Limited effects of beta-endorphin compared to loperamide or fentanyl in a neuroendocrine biomarker assay in non-human primates. Psychoneuroendocrinology\| PubMed ID: 18171605	2008
Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions. Journal of proteome research\| PubMed ID: 18271525	2008
Genomic and proteomic analysis of phiEco32, a novel Escherichia coli bacteriophage. Journal of molecular biology\| PubMed ID: 18294652	2008
Efficient identification of phosphorylation by mass spectrometric phosphopeptide fingerprinting. Analytical chemistry\| PubMed ID: 18321079	2008
A novel high-capacity ion trap-quadrupole tandem mass spectrometer. International journal of mass spectrometry\| PubMed ID: 18347735	2007
Human cytomegalovirus protein UL38 inhibits host cell stress responses by antagonizing the tuberous sclerosis protein complex. Cell host & microbe\| PubMed ID: 18407068	2008
Ku80 脱从 DNA 双链断裂致泛素化。 The Journal of cell biology\| PubMed ID: 18678709	2008
Sequential tyrosine sulfation of CXCR4 by tyrosylprotein sulfotransferases. Biochemistry\| PubMed ID: 18834145	2008
Regulation of class switch recombination and somatic mutation by AID phosphorylation. The Journal of experimental medicine\| PubMed ID: 18838546	2008
High-yield isolation and subcellular proteomic characterization of nuclear and subnuclear structures from trypanosomes. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 18951162	2008
Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature\| PubMed ID: 19098896	2009
Carbon dioxide-induced anesthesia results in a rapid increase in plasma levels of vasopressin. Endocrinology\| PubMed ID: 19213839	2009
Using genetic mouse models to study the biology and pathology of autophagy in the central nervous system. Methods in enzymology\| PubMed ID: 19216906	2009
Distinct regulation of autophagic activity by Atg14L and Rubicon associated with Beclin 1-phosphatidylinositol-3-kinase complex. Nature cell biology\| PubMed ID: 19270693	2009
Rapid sensitive analysis of cysteine rich peptide venom components. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 19380747	2009
Proteomic studies of a single CNS synapse type: the parallel fiber/purkinje cell synapse. PLoS biology\| PubMed ID: 19402746	2009
Evidence for a shared nuclear pore complex architecture that is conserved from the last common eukaryotic ancestor. Molecular & cellular proteomics : MCP\| PubMed ID: 19525551	2009
Identification of SMARCAL1 as a component of the DNA damage response. The Journal of biological chemistry\| PubMed ID: 19841479	2009
Helicobacter pylori CagA inhibits PAR1-MARK family kinases by mimicking host substrates. Nature structural & molecular biology\| PubMed ID: 19966800	2010
Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA. Molecular cell\| PubMed ID: 20005841	2009
GINS motion reveals replication fork progression is remarkably uniform throughout the yeast genome. Molecular systems biology\| PubMed ID: 20212525	2010
Host factors associated with the Sindbis virus RNA-dependent RNA polymerase: role for G3BP1 and G3BP2 in virus replication. Journal of virology\| PubMed ID: 20392851	2010
Human cytomegalovirus pUL83 stimulates activity of the viral immediate-early promoter through its interaction with the cellular IFI16 protein. Journal of virology\| PubMed ID: 20504932	2010
Enhancement of transport selectivity through nano-channels by non-specific competition. PLoS computational biology\| PubMed ID: 20548778	2010
Developmental expression of human hemoglobins mediated by maturation of their subunit interfaces. Protein science : a publication of the Protein Society\| PubMed ID: 20572018	2010
Human cytomegalovirus UL29/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNA. PLoS pathogens\| PubMed ID: 20585571	2010
Protein quantitation using mass spectrometry. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 20835801	2010
Escherichia coli condensin MukB stimulates topoisomerase IV activity by a direct physical interaction. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 20921377	2010
Temporal regulation of gene expression of the Thermus thermophilus bacteriophage P23-45. Journal of molecular biology\| PubMed ID: 21050864	2011
Global analysis of Cdc14 phosphatase reveals diverse roles in mitotic processes. The Journal of biological chemistry\| PubMed ID: 21127052	2011
Amino-terminal phosphorylation of activation-induced cytidine deaminase suppresses c-myc/IgH translocation. Molecular and cellular biology\| PubMed ID: 21135131	2011
Phosphorylation-dependent 14-3-3 binding to LRRK2 is impaired by common mutations of familial Parkinson's disease. PloS one\| PubMed ID: 21390248	2011
A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Molecular & cellular proteomics : MCP\| PubMed ID: 21454883	2011
High-Capacity Ion Trap Coupled to a Time-of-Flight Mass Spectrometer for Comprehensive Linked Scans with no Scanning Losses. International journal of mass spectrometry\| PubMed ID: 21516228	2011
Affinity purification of protein complexes. Cold Spring Harbor protocols\| PubMed ID: 21536765	2011
Conjugation of magnetic beads for immunopurification of protein complexes. Cold Spring Harbor protocols\| PubMed ID: 21536766	2011
Mass spectrometry in the postgenomic era. Annual review of biochemistry\| PubMed ID: 21675917	2011
Sequence and structural convergence of broad and potent HIV antibodies that mimic CD4 binding. Science (New York, N.Y.)\| PubMed ID: 21764753	2011
Cdk1 uncouples CtIP-dependent resection and Rad51 filament formation during M-phase double-strand break repair. The Journal of cell biology\| PubMed ID: 21893598	2011
Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs. The Journal of biological chemistry\| PubMed ID: 22009756	2011
Intrinsic regulation of hemoglobin expression by variable subunit interface strengths. The FEBS journal\| PubMed ID: 22129306	2012
Quantitative Chemical Proteomics Approach To Identify Post-translational Modification-Mediated Protein-Protein Interactions. Journal of the American Chemical Society\| PubMed ID: 22239320	2012
Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization. Genes & development\| PubMed ID: 22241783	2012
Transcriptional regulation by Pol II(G) involving mediator and competitive interactions of Gdown1 and TFIIF with Pol II. Molecular cell\| PubMed ID: 22244332	2012
Proteomics on the rims: insights into the biology of the nuclear envelope and flagellar pocket of trypanosomes. Parasitology\| PubMed ID: 22309600	2012
Structure-function mapping of a heptameric module in the nuclear pore complex. The Journal of cell biology\| PubMed ID: 22331846	2012
RNA ligase RtcB splices 3'-phosphate and 5'-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3')pp(5')G intermediates. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 22474365	2012
Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels. BioTechniques\| PubMed ID: 22668517	2012
Constrained de novo sequencing of conotoxins. Journal of proteome research\| PubMed ID: 22709442	2012
The biochemical anatomy of cortical inhibitory synapses. PloS one\| PubMed ID: 22768092	2012
Strategy for identifying dendritic cell-processed CD4+ T cell epitopes from the HIV gag p24 protein. PloS one\| PubMed ID: 22860026	2012
Activation of DSB processing requires phosphorylation of CtIP by ATR. Molecular cell\| PubMed ID: 23273981	2013
Examining post-translational modification-mediated protein-protein interactions using a chemical proteomics approach. Protein science : a publication of the Protein Society\| PubMed ID: 23281010	2013
Rif1 prevents resection of DNA breaks and promotes immunoglobulin class switching. Science (New York, N.Y.)\| PubMed ID: 23306439	2013
SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action. Cell\| PubMed ID: 23415230	2013
The yeast Snt2 protein coordinates the transcriptional response to hydrogen peroxide-mediated oxidative stress. Molecular and cellular biology\| PubMed ID: 23878396	2013
Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly. Developmental cell\| PubMed ID: 24075807	2013
Supervillin binding to myosin II and synergism with anillin are required for cytokinesis. Molecular biology of the cell\| PubMed ID: 24088567	2013
The nuclear basket proteins Mlp1p and Mlp2p are part of a dynamic interactome including Esc1p and the proteasome. Molecular biology of the cell\| PubMed ID: 24152732	2013
Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition. Cell\| PubMed ID: 24267889	2013
Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity. Molecular biology of the cell\| PubMed ID: 24600046	2014
Sample limited characterization of a novel disulfide-rich venom peptide toxin from terebrid marine snail Terebra variegata. PloS one\| PubMed ID: 24713808	2014
NPC mimics: probing the mechanism of nucleocytoplasmic transport. Methods in cell biology\| PubMed ID: 24857739	2014
Method for identifying phosphorylated substrates of specific cyclin/cyclin-dependent kinase complexes. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 25049391	2014
Molecular architecture and function of the SEA complex, a modulator of the TORC1 pathway. Molecular & cellular proteomics : MCP\| PubMed ID: 25073740	2014
Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. The Journal of biological chemistry\| PubMed ID: 25086043	2014
Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex. Molecular & cellular proteomics : MCP\| PubMed ID: 25139911	2014
Structural characterization by cross-linking reveals the detailed architecture of a coatomer-related heptameric module from the nuclear pore complex. Molecular & cellular proteomics : MCP\| PubMed ID: 25161197	2014
A pipeline for determining protein-protein interactions and proximities in the cellular milieu. Molecular & cellular proteomics : MCP\| PubMed ID: 25172955	2014
A robust pipeline for rapid production of versatile nanobody repertoires. Nature methods\| PubMed ID: 25362362	2014
Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit. Nature structural & molecular biology\| PubMed ID: 25383669	2014
Touching from a distance. Nucleus (Austin, Tex.)\| PubMed ID: 25482119	2014
Maximizing ion transmission from atmospheric pressure into the vacuum of mass spectrometers with a novel electrospray interface. Journal of the American Society for Mass Spectrometry\| PubMed ID: 25588722	2015
Protein kinase A directly phosphorylates metabotropic glutamate receptor 5 to modulate its function. Journal of neurochemistry\| PubMed ID: 25639954	2015
Optimizing Electrospray Interfaces Using Slowly Diverging Conical Duct (ConDuct) Electrodes. Journal of the American Society for Mass Spectrometry\| PubMed ID: 25667060	2015
Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives. BioTechniques\| PubMed ID: 25757543	2015