MALDI Sample Preparation: the Ultra Thin Layer Method

David Fenyo; Qingjun Wang; Jeffrey A. DeGrasse; Julio C. Padovan; Martine Cadene; Brian T. Chait

doi:10.3791/192

MALDI Sample Preparation: the Ultra Thin Layer Method

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Biology

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JoVE Journal Biology

MALDI Sample Preparation: the Ultra Thin Layer Method

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05:28 min

April 29, 2007

DOI: 10.3791/192-v

David Fenyo¹, Qingjun Wang¹, Jeffrey A. DeGrasse¹, Julio C. Padovan¹, Martine Cadene¹, Brian T. Chait¹

¹Laboratory of Mass Spectrometry and Gaseous Ion Chemistry,Rockefeller University

This video demonstrates the preparation of an ultra-thin matrix/analyte layer for analyzing peptides and proteins by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS).

Welcome to the CHI Lab tutorial on the ultra thin layer method, which is particularly useful for the accurate mass determination of proteins. Because of the sensitive nature of mass spectrometry, we only use HPLC grade reagents as well as powder free latex or nitrile gloves To clean the Plate. Rinse alternately with methanol water, and then finally methanol again.

In between each rinse, gently wipe away the excess solvent using lens tissue. You may repeat these wash steps as many times as necessary to produce a clean plate. And once cleaned and dry, the plate should be used Immediately.

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