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MALDI Sample Preparation: the Ultra Thin Layer Method
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MALDI Sample Preparation: the Ultra Thin Layer Method

MALDI Sample Preparation: the Ultra Thin Layer Method

DOI:
10.3791/192-v

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05:28 min

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April 29, 2007

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, , , , ,
1Laboratory of Mass Spectrometry and Gaseous Ion Chemistry,Rockefeller University

Chapters

  • 00:20Step 1: cleaning of the sample plate
  • 00:46Step 2: preparation of the thin layer substrate solution
  • 00:58Step 3: making the thin layer substrate
  • 01:52Step 4: preparation of the matrix solution
  • 02:05Step 5: test of the thin layer
  • 02:50Step 6: sample application
  • 03:47Step 7: calibrant application
  • 04:20Step 8: washing step
  • 05:05Step 9: acquisition of mass spectra

Summary

Automatic Translation

Automatic Translation

This video demonstrates the preparation of an ultra-thin matrix/analyte layer for analyzing peptides and proteins by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS).

Tags

MALDISample PreparationUltra Thin Layer MethodMatrix-Assisted Laser Desorption Ionization Mass SpectrometryMatrix CrystalsAlpha-cyano-4-hydroxycinnamic AcidSubstrate LayerCrystallizationAdvantagesGreater Tolerance To ImpuritiesBetter ResolutionHigher Spatial UniformityAccurate Mass DeterminationMembrane ProteinsIon ChannelsMetabolite TransportersReceptorsTransmembrane DomainsSoluble ProteinsMolecular MassesHigh-quality Spectra

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