a-novel-serum-free-2d-3d-culture-system-comprising-two-small

A Novel Serum-Free 2D and 3D Culture System Comprising Two Small Molecules for Culturing Mouse Lacrimal Gland Epithelial Cells

Lacrimal gland (LG) dysfunction is a major cause of aqueous-deficient dry eye disease, and cell-based and tissue engineering therapies show significant ...

China-Japan Union Hospital of Jilin University

Xiamen University

Meibomian glands (MGs) are crucial for maintaining tear film stability and ocular surface health. Here, we aim to establish a novel organotypic culture model of MGs and explore the risk factors of MG dysfunction (MGD).

Organotypic culture model of mouse meibomian gland as a screening platform for risk factors related to meibomian gland dysfunction.

This study aims to develop a novel serum-free culture strategy containing only two small molecules, Y27632 and SB431542 (2C), for in vitro expansion of mouse lacrimal gland epithelial cells (LGECs) and investigate an innovative therapeutic approach for lacrimal gland (LG) injury.

Distinctive small molecules blend: Promotes lacrimal gland epithelial cell proliferation in vitro and accelerates lacrimal gland injury repair in vivo.

To investigate the differentiation effects of retinoic acid on primary conjunctival epithelium using both 2D and 3D models, and to evaluate its in vivo effects on conjunctival epithelium and goblet cells in mice using a novel goblet cell assessment method.

Retinoic acid promotes conjunctival epithelium differentiation and goblet cell regeneration: evidence from novel 3D conjunctival organoids and whole-mount PAS staining.

Article	Year
Organotypic culture model of mouse meibomian gland as a screening platform for risk factors related to meibomian gland dysfunction. The ocular surface\| PubMed ID: 37619669	2023
Distinctive small molecules blend: Promotes lacrimal gland epithelial cell proliferation in vitro and accelerates lacrimal gland injury repair in vivo. The ocular surface\| PubMed ID: 39209152	2024
Retinoic acid promotes conjunctival epithelium differentiation and goblet cell regeneration: evidence from novel 3D conjunctival organoids and whole-mount PAS staining. The ocular surface\| PubMed ID: 40348334	2025

Baihui Zeng

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