therapeutic-efficacy-308-nm-excimer-laser-combined-with-matrine

Therapeutic Efficacy of 308-nm Excimer Laser Combined with Matrine in Vitiligo by Modulation of Serum Immunoglobulins and Inflammatory Cytokines

Vitiligo is a chronic autoimmune depigmenting disorder characterized by the loss of functional melanocytes and aberrant immune activation. Despite ...

Xingtai People's Hospital

It has been reported that ubiquitin C-terminal hydrolase-L3 (UCHL3) plays an important role in cancer development; however, the role of UCHL3 in melanoma remains unclear. The present study aimed to investigate the role of ubiquitin C-terminal hydrolase-L3 (UCHL3) and determine its underlying molecular mechanisms in melanoma occurrence and development using  studies. Reverse transcription-quantitative PCR analysis was performed to detect UCHL3 mRNA expression. The MTT assay was performed to assess cell proliferation. Cell apoptosis was analyzed via flow cytometry and the TUNEL assay. Cell ultrastructure was observed via transmission electron microscopy. LC3B protein expression was detected via cellular immunofluorescence, while neural precursor cell-expressed developmentally downregulated protein 8 (NEDD8) and LC3 protein expression levels, and NEDD8 ubiquitination were detected via western blot analysis. The results demonstrated that transfection with small interfering (si)RNA-UCHL3 significantly suppressed cell proliferation, whereas apoptosis was significantly enhanced, as well as autophagy, autophagosome formation and LC3B protein expression. In addition, NEDD8 protein expression and autophagosome numbers significantly decreased, while the LC3II/LC3I ratio significantly increased. NEDD8 knockdown via transfection with si-NEDD8 had similar effects to si-UCHL3, as well as si-UCHL3+ si-NEDD8. Taken together, the results of the present study suggest that UCHL3 knockdown decreases melanoma cell proliferation by increasing cell autophagy through regulating NEDD8 expression and autophagosome numbers.

UCHL3 plays an important role in the occurrence and development of melanoma.

The present study aimed to discuss the effects and relative mechanisms of NEDD4 E3 ubiquitin protein ligase (NEDD4) in cutaneous melanoma (CMM) occurrence and development. Clinical cancer and adjacent normal tissues samples were collected to analyze pathological changes and protein expression of NEDD4. Moreover, small interfering (si)RNA was used to knockdown NEDD4 expression in SK-MEL-2 and Malme-3M cells. Cellular proliferation, apoptosis, invasiveness and migration were examined using colony formation, flow cytometric, Transwell and wound-healing assays, respectively. In addition, the relative mRNA and protein expression levels of NEDD4, notch receptor 1 (Notch1) and PTEN were evaluated via reverse transcription-quantitative (RT-q) PCR and western blotting. It was found that NEDD4 mRNA and protein expression were significantly upregulated (both P<0.01). Following NEDD4-knockdown, colony number was significantly decreased, while the apoptotic rate was significantly increased, the invasive cell number was significantly inhibited and the wound-healing capacity was significantly decreased. Following si-NEDD4 transfection, RT-qPCR and western blotting revealed that NEDD4 and Notch1 mRNA and protein expression levels were significantly downregulated, while those of PTEN were significantly upregulated in the SK-MEL-2 and Malme-3M cell lines. Collectively, the current results suggest that NEDD4-knockdown effectively suppressed CMM biological activity by regulating the Notch1/PTEN pathway .

NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development.

The relationship between the systemic immune-inflammation index (SII) and disease activity in rheumatoid arthritis (RA) remains unclear. This study aimed to clarify this association in a cohort of hospitalized RA patients overwhelmingly with moderate-to-high disease activity. We consecutively included 1,191 RA patients hospitalized at Xingtai People's Hospital from March 2022 to December 2024. SII was calculated as (neutrophils × platelets)/lymphocytes. Disease activity was assessed using the Disease Activity Score 28-erythrocyte sedimentation rate (DAS28ESR) and C-reactive protein (DAS28-CRP). Multiple linear regression and generalized additive models were employed to explore the association between SII and disease activity. For regression analysis, SII underwent natural logarithm transformation to improve normality. After strict eligibility criteria, 997 individuals (mean age 57.66 years; 77.93% female, 95.6% moderate-to-high disease activity) were included for the final analysis. A nonlinear relationship was identified with a threshold at LnSII = 5.56 (SII = 260.36). Above this cutoff, LnSII showed positive correlations with both DAS28-ESR (β = 0.53, 95% CI: 0.44-0.63) and DAS28-CRP (β = 0.63, 95% CI: 0.53-0.72). No significant association was found below this threshold (DAS28-ESR: β = -0.56, 95% CI: -1.13- 0.02; DAS28-CRP: β = -0.35, 95% CI: -0.91-0.20). These findings suggest SII levels were independently positive associated with DAS28-ESR and DAS28-CRP among RA patients with SII > 260.36, supporting its potential as a useful biomarker for evaluating disease activity in RA patients with predominantly moderate-to-high disease activity.

Article	Year
UCHL3 plays an important role in the occurrence and development of melanoma. Oncology letters\| PubMed ID: 34539860	2021
NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development. Experimental and therapeutic medicine\| PubMed ID: 34650630	2021
Association between systemic immune-inflammation index and disease activity score 28 in patients with rheumatoid arthritis: a cross-sectional study. Scientific reports\| PubMed ID: 41413101	2025

Lihui An

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