therapeutic-efficacy-308-nm-excimer-laser-combined-with-matrine

Therapeutic Efficacy of 308-nm Excimer Laser Combined with Matrine in Vitiligo by Modulation of Serum Immunoglobulins and Inflammatory Cytokines

Vitiligo is a chronic autoimmune depigmenting disorder characterized by the loss of functional melanocytes and aberrant immune activation. Despite ...

Xingtai People's Hospital

Non-tuberculous mycobacteria (NTM) can cause various respiratory diseases and even death in severe cases, and its incidence has increased rapidly worldwide. To date, it's difficult to use routine diagnostic methods and strain identification to precisely diagnose various types of NTM infections. We combined systematic comparative genomics with machine learning to select new diagnostic markers for precisely identifying five common pathogenic NTMs (Mycobacterium kansasii, Mycobacterium avium, Mycobacterium intracellular, Mycobacterium chelonae, Mycobacterium abscessus). A panel including six genes and two SNPs (nikA, benM, codA, pfkA2, mpr, yjcH, rrl C2638T, rrl A1173G) was selected to simultaneously identify the five NTMs with high accuracy (> 90%). Notably, the panel only containing the six genes also showed a good classification effect (accuracy > 90%). Additionally, the two panels could precisely differentiate the five NTMs from M. tuberculosis (accuracy > 99%). We also revealed some new marker genes/SNPs/combinations to accurately discriminate any one of the five NTMs separately, which provided the possibility to diagnose one certain NTM infection precisely. Our research not only reveals novel promising diagnostic markers to promote the development of precision diagnosis in NTM infectious, but also provides an insight into precisely identifying various genetically close pathogens through comparative genomics and machine learning.

Combining comparative genomic analysis with machine learning reveals some promising diagnostic markers to identify five common pathogenic non-tuberculous mycobacteria.

The present study aimed to discuss the effects and relative mechanisms of NEDD4 E3 ubiquitin protein ligase (NEDD4) in cutaneous melanoma (CMM) occurrence and development. Clinical cancer and adjacent normal tissues samples were collected to analyze pathological changes and protein expression of NEDD4. Moreover, small interfering (si)RNA was used to knockdown NEDD4 expression in SK-MEL-2 and Malme-3M cells. Cellular proliferation, apoptosis, invasiveness and migration were examined using colony formation, flow cytometric, Transwell and wound-healing assays, respectively. In addition, the relative mRNA and protein expression levels of NEDD4, notch receptor 1 (Notch1) and PTEN were evaluated via reverse transcription-quantitative (RT-q) PCR and western blotting. It was found that NEDD4 mRNA and protein expression were significantly upregulated (both P<0.01). Following NEDD4-knockdown, colony number was significantly decreased, while the apoptotic rate was significantly increased, the invasive cell number was significantly inhibited and the wound-healing capacity was significantly decreased. Following si-NEDD4 transfection, RT-qPCR and western blotting revealed that NEDD4 and Notch1 mRNA and protein expression levels were significantly downregulated, while those of PTEN were significantly upregulated in the SK-MEL-2 and Malme-3M cell lines. Collectively, the current results suggest that NEDD4-knockdown effectively suppressed CMM biological activity by regulating the Notch1/PTEN pathway .

NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development.

To investigate the effect of phototherapy combined with melanocyte transplantation on the activity index of vitiligo.

Article	Year
Combining comparative genomic analysis with machine learning reveals some promising diagnostic markers to identify five common pathogenic non-tuberculous mycobacteria. Microbial biotechnology\| PubMed ID: 34019733	2021
NEDD4 E3 ubiquitin protein ligase serves an important role in cutaneous melanoma occurrence and development. Experimental and therapeutic medicine\| PubMed ID: 34650630	2021
Impact of Combined Phototherapy and Melanocyte Transplantation on Indicators of Vitiligo Activity. Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]\| PubMed ID: 38984521	2024
Two novel mutations in LSS gene associated with hypotrichosis simplex in a Chinese family. Journal of cosmetic dermatology\| PubMed ID: 39152648	2024

Linfang Yang

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