gastrodin-alleviates-tau-hyperphosphorylation-associated-with-aktgsk

Gastrodin Alleviates Tau Hyperphosphorylation Associated with AKT/GSK-3&#946; Signaling Changes in an Alzheimer's Disease Cell Model

Alzheimer&#39;s disease (AD) is a common neurodegenerative disease for which there are currently limited effective drugs. As the principal bioactive ...

Zhejiang Sci-Tech University

Zhejiang Sci-Tech University Shaoxing Academy of Biomedicine

Based on our previous research on the specific miRNAs identified from Gastrodia elata, we selected Gas-miR2-3p to investigate its effects on neuroinflammation via in vitro and in vivo experiments.

 specific miRNA attenuates neuroinflammation via modulating NF-κB signaling pathway.

Alzheimer's disease (AD) is currently the most common neurodegenerative disease. Glycogen synthase kinase 3β (GSK-3β) is a pivotal factor in AD pathogenesis. Recent research has demonstrated that plant miRNAs exert cross-kingdom regulation on the target genes in animals.  () is a valuable traditional Chinese medicine that has significant pharmacological activity against diseases of the central nervous system (CNS). Our previous studies have indicated that -specific miRNA plays a cross-kingdom regulatory role for the NF-B signaling pathway in mice. In this study, further bioinformatics analysis suggested that Gas-miR36-5p targets GSK-3β. Through western blot, RT-qPCR, and assessments of T-AOC, SOD, and MDA levels, Gas-miR36-5p demonstrated its neuroprotective effects in an AD cell model. Furthermore, Gas-miR36-5p was detected in the murine brain tissues. The results of the Morris water maze test and western blot analysis provided positive evidence for reversing the learning deficits and hyperphosphorylation of Tau in AD mice, elucidating significant neuroprotective effects in an AD model following  RNA administration. Our research emphasizes Gas-miR36-5p as a novel -specific miRNA with neuroprotective properties in Alzheimer's disease by targeting GSK-3β. Consequently, our findings provide valuable insights into the cross-kingdom regulatory mechanisms underlying -specific miRNA, presenting a novel perspective for the treatment of Alzheimer's disease.

Article	Year
specific miRNA attenuates neuroinflammation via modulating NF-κB signaling pathway. The International journal of neuroscience\| PubMed ID: 37965801	2024
Neuroprotection and Mechanism of Gas-miR36-5p from in an Alzheimer's Disease Model by Regulating Glycogen Synthase Kinase-3β. International journal of molecular sciences\| PubMed ID: 38139125	2023

Xiaoqi Wu

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