a-bioactive-component-from-aconitum-pendulum-attenuates-rheumatoid

A Bioactive Component from <em>Aconitum pendulum</em> Attenuates Rheumatoid Arthritis-Related Inflammation in Rats via PI3K/Akt Pathway Regulation

In traditional Chinese medicine and various ethnic medical practices, the roots of Aconitum pendulum N. Busch are commonly used for their ability to ...

Qinghai minzu university

Ten compounds were isolated from the 95% ethanol extract of the roots of Aconitum pendulum using silica gel chromatography, gel chromatography, and semi-preparative high-performance liquid chromatography. Their structures were identified by various spectroscopic methods, including UV, IR, HR-ESI-MS, and NMR. The compounds were identified as two phenolic compounds: 4-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyloxy]-3-methoxypropiophenone(1) and corchoionoside C(2); two lignans:(+)-oxabicyclooctalignan(3) and(-)-oxabicyclooctalignan(4); five diterpenoid alkaloids: benzoylaconine(5), 14-benzoyl-8-O-methylaconine(6),(-)-(A-b)-14α-benzoyloxy-N-ethyl-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxyaconitane(7), smirnotine A(8), and aconitine(9); and one coumarin compound: scopoletin(10). Among them, compound 1 is a novel phenolic glycoside, while compounds 3, 4, 7, 8, and 10 were isolated from A. pendulum for the first time. The anti-inflammatory activities of the compounds were evaluated using an LPS-induced RAW264.7 macrophage inflammation model. Compounds 5, 6, and 10 inhibited LPS-induced nitric oxide(NO) production in the cells, with IC_(50) values of(34.1±1.3),(28.0±1.0), and(20.3±1.7) μmol·L~(-1), respectively.

Si-Qi Chen

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