a-bioactive-component-from-aconitum-pendulum-attenuates-rheumatoid

A Bioactive Component from <em>Aconitum pendulum</em> Attenuates Rheumatoid Arthritis-Related Inflammation in Rats via PI3K/Akt Pathway Regulation

In traditional Chinese medicine and various ethnic medical practices, the roots of Aconitum pendulum N. Busch are commonly used for their ability to ...

Qinghai minzu university

Qinghai Modern Plateau Biomedical Industry Institute

Qinghai Provincial Key Laboratory of Plant Resource Chemistry on the Tibetan Plateau

This study aims to establish a method for the simultaneous determination of 7 active components in Dracocephalum tanguticum and to evaluate the quality of medicinal materials from different habitats. The method was established with high performance liquid chromatography(HPLC) and the gradient elution was performed with the mobile phase of acetonitrile-methanol-0.2% phosphoric acid solution at a column temperature of 35 ℃, an injection volume of 15 μL, and a flow rate of 0.6 mL·min~(-1). The detection wavelength was set as 215 nm. With rosmarinic acid as the internal reference, the relative correction factors and the content of other 6 components were calculated. The results were compared with those obtained with the external standard method. The results showed that the samples from Huangzhong county, Qinghai province had the best quality, with the highest content of p-hydroxybenzoic acid, cosmosiin, rosmarinic acid, oleanolic acid, and ursolic acid(9.29, 12.14, 6.02, 3.11, 17.67 mg·g~(-1) respectively). The samples from Chaya county, Tibet autonomous region ranked the second, with the highest content of betulin and betulinic acid(15.53, 7.17 mg·g~(-1), respectively). The method is accurate, reliable, and repeatable and suitable for the simultaneous determination of multiple components in D. tanguticum. The content of functional components varied in the samples from different producing areas and can be used as the indicator for the quality evaluation of medicinal materials.

[Multi-component content determination of Dracocephalum tanguticum by quantitative analysis of multi-components by single-marker].

Eleutherine bulbosa (Mill.) Urb. is a medicinal and edible plant with various benefits for humans and animals. In this work, four new phenolic constituents (1-4), along with six known phenolic compounds (5-10) were obtained from the red bulbs of E. bulbosa. Their structures with absolute configurations were characterized by extensive spectroscopic analysis, combined with HR-ESI-MS and quantum mechanical electronic circular dichroism (ECD). Compounds 1 and 2 are novel homologous and heterodimers, respectively, featuring an unusual spiro ring system. All isolated phenolic constituents were tested for their antibacterial effects. The results revealed four phenolic compounds 1-3 and 7 showed moderate antibacterial activity against Bacillus subtilis, Staphylococcus aureus and Escherichia coli with minimum inhibitory concentration (MIC) values ranging from 15.6 to 250.0 μg/mL.

Phenolic constituents with antibacterial activity from Eleutherine bulbosa.

Two new phenolic compounds aconitol A () and B (), together with three known compounds including one phenolic constituent () and two diterpenoid alkaloids (-), were isolated from the roots of . Their structures were assigned by HRESIMS, extensive spectral methods and compared with literature data. All obtained compounds (-) were tested for their inhibitory activity against NO production in LPS-activated RAW 264.7 macrophages. Compound  and  showed moderate inhibitory effects.

Two new phenolic constituents from the  Busch roots.

Traditional Chinese medicine quality control faces challenges, lacking multidimensionality and reliable quantitative evidence. Comprehensive evaluation models based on external characteristics and multiple indicator metabolites are the future research direction. This study focuses on  slices, aiming to establish a method for its quality evaluation.

Quality evaluation of  slices from different regions based on appearance traits and multi-index metabolites combined with TOPSIS and gray relation analysis.

Three novel meroterpenoids with unique benzo-fused 10-membered carbocycle skeletons, named tricholactones A-C (1-3), along with four known cyclic dipeptides (4-7), were isolated from the fungus Tricholoma sp. HD0815-7. Their chemical structures and absolute configurations were characterized by comprehensive analysis of HR-ESI-MS, 1D- and 2D-NMR and quantum mechanical electronic circular dichroism (ECD). Compounds 1-3 are novel natural meroterpenoids containing terpenoid scaffolds, phenols units and amino acid residues. Moreover, compound 3 features an unprecedented pentacyclic ring system (8/6/10/5/3) among these 10-membered carbocycle meroterpenoids. All isolated compounds 1-7 were tested for their cytotoxic activity. The results revealed that compound 2 exhibited strong cytotoxicity activity against HGC-27 cells with IC values of 2.34 ± 0.32 μM.

Three novel meroterpenoids with unique benzo-fused 10-membered carbocycle skeletons from the fungus Tricholoma sp. HD0815-7.

Ten compounds were isolated from the 95% ethanol extract of the roots of Aconitum pendulum using silica gel chromatography, gel chromatography, and semi-preparative high-performance liquid chromatography. Their structures were identified by various spectroscopic methods, including UV, IR, HR-ESI-MS, and NMR. The compounds were identified as two phenolic compounds: 4-[α-L-arabinofuranosyl-(1→6)-β-D-glucopyranosyloxy]-3-methoxypropiophenone(1) and corchoionoside C(2); two lignans:(+)-oxabicyclooctalignan(3) and(-)-oxabicyclooctalignan(4); five diterpenoid alkaloids: benzoylaconine(5), 14-benzoyl-8-O-methylaconine(6),(-)-(A-b)-14α-benzoyloxy-N-ethyl-13β,15α-dihydroxy-1α,6α,8β,16β,18-pentamethoxyaconitane(7), smirnotine A(8), and aconitine(9); and one coumarin compound: scopoletin(10). Among them, compound 1 is a novel phenolic glycoside, while compounds 3, 4, 7, 8, and 10 were isolated from A. pendulum for the first time. The anti-inflammatory activities of the compounds were evaluated using an LPS-induced RAW264.7 macrophage inflammation model. Compounds 5, 6, and 10 inhibited LPS-induced nitric oxide(NO) production in the cells, with IC_(50) values of(34.1±1.3),(28.0±1.0), and(20.3±1.7) μmol·L~(-1), respectively.

[A new phenolic glycoside from roots of Aconitum pendulum].

: Chloroplast genomes are essential for understanding the systematics and adaptive evolution of alpine plants, yet genomic data for high-altitude  species remain scarce. , a medicinal plant endemic to the Qinghai-Tibet Plateau, exhibits notable high-altitude adaptations, but its plastome features and evolutionary position are still unclear. This study aims to assemble and characterize its complete chloroplast genome and clarify its phylogenetic placement within . : Using Illumina NovaSeq data, we de novo assembled the  plastome, annotated it with CPGAVAS2, and compared it with 12 published Ranunculaceae plastomes. We analyzed IR-boundary dynamics, genome-wide sequence variation, and codon-usage bias and constructed a maximum-likelihood phylogeny based on 69 shared protein-coding genes. : The plastome is 154,161 bp (GC 38.24%) with a canonical quadripartite structure, encoding 131 genes (87 CDS, 8 rRNA, 37 tRNA). An IR expansion into the SSC region yields the shortest SSC reported among the compared  species and produces unique structural variants. Photosynthetic genes are extremely conserved (nucleotide diversity Pi ≤ 0.01), whereas several loci (e.g.,  and ) are highly divergent (Pi ≥ 0.05). Codon usage shows a strong bias toward AU-ending triplets. Phylogenetically,  forms a 100%-bootstrap clade with other high-altitude congeners, supporting the non-monophyly of . : This study delineates the plastome architecture and putative adaptive signatures of , identifies robust candidate loci for DNA barcoding, and provides molecular evidence for taxonomic revision and conservation strategies in .

Article	Year
[Multi-component content determination of Dracocephalum tanguticum by quantitative analysis of multi-components by single-marker]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica\| PubMed ID: 37802794	2023
Phenolic constituents with antibacterial activity from Eleutherine bulbosa. Fitoterapia\| PubMed ID: 38122855	2024
Two new phenolic constituents from the Busch roots. Natural product research\| PubMed ID: 39887256	2026
Quality evaluation of slices from different regions based on appearance traits and multi-index metabolites combined with TOPSIS and gray relation analysis. Frontiers in pharmacology\| PubMed ID: 40432901	2025
Three novel meroterpenoids with unique benzo-fused 10-membered carbocycle skeletons from the fungus Tricholoma sp. HD0815-7. Fitoterapia\| PubMed ID: 41485686	2026
[A new phenolic glycoside from roots of Aconitum pendulum]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica\| PubMed ID: 41508076	2025
Assembly of the Chloroplast Genome and Comparative Genomics Within . Genes\| PubMed ID: 41751624	2026

Min Ma

Biography

JoVE Journal Publications

Other Publications