Christoph H. Borchers

University of Victoria-Genome BC Proteomics Centre, University of Victoria

Affiliated withUniversity of Victoria University of Victoria-Genome BC Proteomics Centre McGill University

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JoVE Journal Publications

ArticleTotal : 2	Year
Publication title Cited by 3	2013
Publication title Cited by 13	2017

Other Publications

Article	Year
Identification of components of protein complexes using a fluorescent photo-cross-linker and mass spectrometry. Analytical chemistry\| PubMed ID: 12033289	2002
Direct MALDI-MS/MS of phosphopeptides affinity-bound to immobilized metal ion affinity chromatography beads. Analytical chemistry\| PubMed ID: 12139050	2002
3' end processing of Drosophila melanogaster histone pre-mRNAs: requirement for phosphorylated Drosophila stem-loop binding protein and coevolution of the histone pre-mRNA processing system. Molecular and cellular biology\| PubMed ID: 12192062	2002
Pseudo-MS3 in a MALDI orthogonal quadrupole-time of flight mass spectrometer. Journal of the American Society for Mass Spectrometry\| PubMed ID: 12322951	2002
Effects of TCDD upon IkappaB and IKK subunits localized in microsomes by proteomics. Archives of biochemistry and biophysics\| PubMed ID: 12361703	2002
Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, a... Molecular and cellular biology\| PubMed ID: 12588979	2003
Mnd2 and Swm1 are core subunits of the Saccharomyces cerevisiae anaphase-promoting complex. The Journal of biological chemistry\| PubMed ID: 12609981	2003
Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes & development\| PubMed ID: 12629047	2003
DNA binding by yeast Mlh1 and Pms1: implications for DNA mismatch repair. Nucleic acids research\| PubMed ID: 12682353	2003
Matrix-assisted laser desorption/ionization directed nano-electrospray ionization tandem mass spectrometric analysis for protein identification. Rapid communications in mass spectrometry : RCM\| PubMed ID: 12876682	2003
Rapid and sensitive identification of epitope-containing peptides by direct matrix-assisted laser desorption/ionization tandem mass spectrometry of peptides affinity-bound to antibody beads. Journal of the American Society for Mass Spectrometry\| PubMed ID: 14530088	2003
Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells. The Journal of cell biology\| PubMed ID: 14970194	2004
Development of a protein chip: a MS-based method for quantitation of protein expression and modification levels using an immunoaffinity approach. Analytical chemistry\| PubMed ID: 15253646	2004
Multi-kinase phosphorylation of the APC/C activator Cdh1 revealed by mass spectrometry. Cell cycle (Georgetown, Tex.)\| PubMed ID: 15467459	2004
Fourier transform ion cyclotron resonance MS reveals the presence of a water molecule in an enzyme transition-state analogue complex. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 15494437	2004
Electrospray ionization tandem mass spectrometry of model peptides reveals diagnostic fragment ions for protein ubiquitination. Rapid communications in mass spectrometry : RCM\| PubMed ID: 15655800	2005
Isotopically coded cleavable cross-linker for studying protein-protein interaction and protein complexes. Molecular & cellular proteomics : MCP\| PubMed ID: 15901824	2005
Identification of components of protein complexes. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 15917630	2005
Mass spectrometric determination of protein ubiquitination. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 15917631	2005
Phosphorylation of the RGS protein Sst2 by the MAP kinase Fus3 and use of Sst2 as a model to analyze determinants of substrate sequence specificity. Biochemistry\| PubMed ID: 15924435	2005
Improved protein identification through the use of unstained gels. Journal of proteome research\| PubMed ID: 15952747	2005
Phosphatase-directed phosphorylation-site determination: a synthesis of methods for the detection and identification of phosphopeptides. Journal of proteome research\| PubMed ID: 16212415	2005
Histone demethylation by a family of JmjC domain-containing proteins. Nature\| PubMed ID: 16362057	2006
Combined top-down and bottom-up proteomics identifies a phosphorylation site in stem-loop-binding proteins that contributes to high-affinity RNA binding. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 16492733	2006
Absolute quantitation of cancer-related proteins using an MS-based peptide chip. BioTechniques\| PubMed ID: 16528910	2005
Cohesin protein SMC1 represses the nuclear receptor CAR-mediated synergistic activation of a human P450 gene by xenobiotics. The Biochemical journal\| PubMed ID: 16623664	2006
Discovery of a small-molecule HIV-1 integrase inhibitor-binding site. Proceedings of the National Academy of Sciences of the United States of America\| PubMed ID: 16785440	2006
Photoaffinity labeling combined with mass spectrometric approaches as a tool for structural proteomics. Expert review of proteomics\| PubMed ID: 16901199	2006
Combining fluorescence detection and mass spectrometric analysis for comprehensive and quantitative analysis of redox-sensitive cysteines in native membrane proteins. Analytical chemistry\| PubMed ID: 17134128	2006
Inhibition of APCCdh1 activity by Cdh1/Acm1/Bmh1 ternary complex formation. The Journal of biological chemistry\| PubMed ID: 17178718	2007
2-methoxyestradiol inhibits the anaphase-promoting complex and protein translation in human breast cancer cells. Cancer research\| PubMed ID: 17234781	2007
Direct interaction with filamins modulates the stability and plasma membrane expression of CFTR. The Journal of clinical investigation\| PubMed ID: 17235394	2007
Identification of covalent modifications in P450 2E1 by 1,2-epoxy-3-butene in vitro. Chemico-biological interactions\| PubMed ID: 17298833	2007
A systems-biology analysis of feedback inhibition in the Sho1 osmotic-stress-response pathway. Current biology : CB\| PubMed ID: 17363249	2007
Mitotic phosphorylation of the anaphase-promoting complex inhibitory subunit Mnd2 is necessary for efficient progression through meiosis i. The Journal of biological chemistry\| PubMed ID: 17459880	2007
Protein arrays for biomarker discovery. Current opinion in molecular therapeutics\| PubMed ID: 17608019	2007
A surface-focused biotinylation procedure identifies the Yersinia pestis catalase KatY as a membrane-associated but non-surface-located protein. Applied and environmental microbiology\| PubMed ID: 17644638	2007
Novel neutrophil-derived proteins in bronchoalveolar lavage fluid indicate an exaggerated inflammatory response in pediatric cystic fibrosis patients. Clinical chemistry\| PubMed ID: 17702859	2007
An immunoaffinity tandem mass spectrometry (iMALDI) assay for detection of Francisella tularensis. Analytica chimica acta\| PubMed ID: 18022413	2007
Mass spectrometric determination of protein ubiquitination. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 18373253	2008
Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry. Proteomics\| PubMed ID: 18655049	2008
Electron capture dissociation of electrosprayed protein ions for spatially resolved hydrogen exchange measurements. Journal of the American Chemical Society\| PubMed ID: 18686958	2008
Data Self-Recalibration and Mixture Mass Fingerprint Searching (DASER-MMF) to enhance protein identification within complex mixtures. Journal of the American Society for Mass Spectrometry\| PubMed ID: 18708289	2008
BiPS, a photocleavable, isotopically coded, fluorescent cross-linker for structural proteomics. Molecular & cellular proteomics : MCP\| PubMed ID: 18838738	2009
Towards high-throughput metabolomics using ultrahigh-field Fourier transform ion cyclotron resonance mass spectrometry. Metabolomics : Official journal of the Metabolomic Society\| PubMed ID: 19081807	2008
Quantitative iTRAQ proteome and comparative transcriptome analysis of elicitor-induced Norway spruce (Picea abies) cells reveals elements of calcium signaling in the early conifer defense re... Proteomics\| PubMed ID: 19105170	2009
A human proteome detection and quantitation project. Molecular & cellular proteomics : MCP\| PubMed ID: 19131327	2009
Top-down proteomics on a high-field Fourier transform ion cyclotron resonance mass spectrometer. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 19241035	2009
Multiple reaction monitoring-based, multiplexed, absolute quantitation of 45 proteins in human plasma. Molecular & cellular proteomics : MCP\| PubMed ID: 19411661	2009
A comparison of MS/MS-based, stable-isotope-labeled, quantitation performance on ESI-quadrupole TOF and MALDI-TOF/TOF mass spectrometers. Proteomics\| PubMed ID: 19504495	2009
Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma. Nature biotechnology\| PubMed ID: 19561596	2009
Accurate quantitation of standard peptides used for quantitative proteomics. Proteomics\| PubMed ID: 19637239	2009
Hydrogen/deuterium exchange mass spectrometry with top-down electron capture dissociation for characterizing structural transitions of a 17 kDa protein. Journal of the American Chemical Society\| PubMed ID: 19670873	2009
Targeted proteomics using selected reaction monitoring reveals the induction of specific terpene synthases in a multi-level study of methyl jasmonate-treated Norway spruce (Picea abies). The Plant journal : for cell and molecular biology\| PubMed ID: 19754519	2009
Use of a combination of isotopically coded cross-linkers and isotopically coded N-terminal modification reagents for selective identification of inter-peptide crosslinks. Analytical chemistry\| PubMed ID: 20050626	2010
ICC-CLASS: isotopically-coded cleavable crosslinking analysis software suite. BMC bioinformatics\| PubMed ID: 20109223	2010
Metabolomics: towards understanding host-microbe interactions. Future microbiology\| PubMed ID: 20143941	2010
Towards the development of an immuno MALDI (iMALDI) mass spectrometry assay for the diagnosis of hypertension. Journal of the American Society for Mass Spectrometry\| PubMed ID: 20199871	2010
Top-down analysis of recombinant histone H3 and its methylated analogs by ESI/FT-ICR mass spectrometry. Proteomics\| PubMed ID: 20486121	2010
Proteomic signatures in plasma during early acute renal allograft rejection. Molecular & cellular proteomics : MCP\| PubMed ID: 20501940	2010
Mass-spectrometry-based clinical proteomics--a review and prospective. The Analyst\| PubMed ID: 20520858	2010
Assay development for the determination of phosphorylation stoichiometry using multiple reaction monitoring methods with and without phosphatase treatment: application to breast cancer signa... Analytical chemistry\| PubMed ID: 20524616	2010
An isotopically coded CID-cleavable biotinylated cross-linker for structural proteomics. Molecular & cellular proteomics : MCP\| PubMed ID: 20622150	2011
A quantitative study of the effects of chaotropic agents, surfactants, and solvents on the digestion efficiency of human plasma proteins by trypsin. Journal of proteome research\| PubMed ID: 20722421	2010
Crosslinking combined with mass spectrometry for structural proteomics. Mass spectrometry reviews\| PubMed ID: 20730915	2010
Characterizing short-lived protein folding intermediates by top-down hydrogen exchange mass spectrometry. Analytical chemistry\| PubMed ID: 20849085	2010
Improving the biomarker pipeline. Clinical chemistry\| PubMed ID: 20940327	2010
Identification of Leishmania-specific protein phosphorylation sites by LC-ESI-MS/MS and comparative genomics analyses. Proteomics\| PubMed ID: 20960452	2010
Mass spectrometry-based technologies for high-throughput metabolomics. Bioanalysis\| PubMed ID: 21083110	2009
Development of an immuno tandem mass spectrometry (iMALDI) assay for EGFR diagnosis. Proteomics. Clinical applications\| PubMed ID: 21136662	2007
Technology development: an overview. Molecular & cellular proteomics : MCP\| PubMed ID: 21193538	2011
Effect of antibiotic treatment on the intestinal metabolome. Antimicrobial agents and chemotherapy\| PubMed ID: 21282433	2011
Impact of salmonella infection on host hormone metabolism revealed by metabolomics. Infection and immunity\| PubMed ID: 21321075	2011
An integrated genomic, proteomic and biochemical analysis of (+)-3-carene biosynthesis in Sitka spruce (Picea sitchensis) genotypes that are resistant or susceptible to white pine weevil. The Plant journal : for cell and molecular biology\| PubMed ID: 21323772	2011
The Saccharomyces cerevisiae fermentation stress response protein Igd1p/Yfr017p regulates glycogen levels by inhibiting the glycogen debranching enzyme. FEMS yeast research\| PubMed ID: 21585652	2011
Mass spectrometric analysis and mutagenesis predict involvement of multiple cysteines in redox regulation of the skeletal muscle ryanodine receptor ion channel complex. Research and reports in biology\| PubMed ID: 21603587	2011
Conformer-specific hydrogen exchange analysis of Aβ(1-42) oligomers by top-down electron capture dissociation mass spectrometry. Analytical chemistry\| PubMed ID: 21635007	2011
The human proteome project: current state and future direction. Molecular & cellular proteomics : MCP\| PubMed ID: 21742803	2011
Metabolomics reveals phospholipids as important nutrient sources during Salmonella growth in bile in vitro and in vivo. Journal of bacteriology\| PubMed ID: 21764918	2011
The deubiquitinase activity of the Salmonella pathogenicity island 2 effector, SseL, prevents accumulation of cellular lipid droplets. Infection and immunity\| PubMed ID: 21875964	2011
Emerging mass spectrometry-based technologies for analyses of chromatin changes: analysis of histones and histone modifications. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 21898261	2011
High-flow multiplexed MRM-based analysis of proteins in human plasma without depletion or enrichment. Clinics in laboratory medicine\| PubMed ID: 21907103	2011
Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles). Molecular & cellular proteomics : MCP\| PubMed ID: 22052993	2011
Recommendations for Mass Spectrometry Data Quality Metrics for Open Access Data (Corollary to the Amsterdam Principles). Journal of proteome research\| PubMed ID: 22053864	2012
Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles). Proteomics\| PubMed ID: 22069307	2012
MeCP2 binds to nucleosome free (linker DNA) regions and to H3K9/H3K27 methylated nucleosomes in the brain. Nucleic acids research\| PubMed ID: 22144686	2012
The use of multiplexed MRM for the discovery of biomarkers to differentiate iron-deficiency anemia from anemia of inflammation. Journal of proteomics\| PubMed ID: 22146476	2012
Dietary patterns and ethnicity are associated with distinct plasma proteomic groups. The American journal of clinical nutrition\| PubMed ID: 22205312	2012
Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles). Proteomics. Clinical applications\| PubMed ID: 22213554	2011
Multiplexed quantification of 63 proteins in human urine by multiple reaction monitoring-based mass spectrometry for discovery of potential bladder cancer biomarkers. Journal of proteomics\| PubMed ID: 22236518	2012
Quantitation of spatially-localized proteins in tissue samples using MALDI-MRM imaging. Analytical chemistry\| PubMed ID: 22356211	2012
Repression of Salmonella enterica phoP expression by small molecules from physiological bile. Journal of bacteriology\| PubMed ID: 22366421	2012
Duplexed iMALDI for the detection of angiotensin I and angiotensin II. Methods (San Diego, Calif.)\| PubMed ID: 22387391	2012
Use of proteinase K nonspecific digestion for selective and comprehensive identification of interpeptide cross-links: application to prion proteins. Molecular & cellular proteomics : MCP\| PubMed ID: 22438564	2012
Structure and dynamics of small soluble Aβ(1-40) oligomers studied by top-down hydrogen exchange mass spectrometry. Biochemistry\| PubMed ID: 22486153	2012
Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia. Nature medicine\| PubMed ID: 22504483	2012
A role for ubiquitinylation and the cytosolic proteasome in turnover of mitochondrial uncoupling protein 1 (UCP1). Biochimica et biophysica acta\| PubMed ID: 22531154	2012
Comparison of standard- and nano-flow liquid chromatography platforms for MRM-based quantitation of putative plasma biomarker proteins. Analytical and bioanalytical chemistry\| PubMed ID: 22547352	2012
MRM-based multiplexed quantitation of 67 putative cardiovascular disease biomarkers in human plasma. Proteomics\| PubMed ID: 22577024	2012
Mass spectrometry-based structural proteomics. European journal of mass spectrometry (Chichester, England)\| PubMed ID: 22641729	2012
Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308. Molecular microbiology\| PubMed ID: 22690807	2012
The effect of pre-analytical variability on the measurement of MRM-MS-based mid- to high-abundance plasma protein biomarkers and a panel of cytokines. PloS one\| PubMed ID: 22701622	2012
Multiple reaction monitoring (MRM): principles and application to coronary artery disease. Circulation. Cardiovascular genetics\| PubMed ID: 22715283	2012
Association between the plasma proteome and serum ascorbic acid concentrations in humans. The Journal of nutritional biochemistry\| PubMed ID: 22841398	2013
Mass Spectrometry in High-Throughput Clinical Biomarker Assays: Multiple Reaction Monitoring. Topics in current chemistry\| PubMed ID: 22886709	2012
Plasma proteomics can discriminate isolated early from dual responses in asthmatic individuals undergoing an allergen inhalation challenge. Proteomics. Clinical applications\| PubMed ID: 22930592	2012
Dithranol as a MALDI matrix for tissue imaging of lipids by Fourier transform ion cyclotron resonance mass spectrometry. Analytical chemistry\| PubMed ID: 22931516	2012
Glutathione S-transferase (GST) M1, but not GSTT1, genotype influences plasma proteomic profiles in Caucasian and East Asian young adults. Journal of proteome research\| PubMed ID: 22946785	2012
Using multiple structural proteomics approaches for the characterization of prion proteins. Journal of proteomics\| PubMed ID: 23085224	2013
Association between the plasma proteome and plasma α-tocopherol concentrations in humans. The Journal of nutritional biochemistry\| PubMed ID: 23199695	2013
Multiplexed quantitation of endogenous proteins in dried blood spots by multiple reaction monitoring-mass spectrometry. Molecular & cellular proteomics : MCP\| PubMed ID: 23221968	2013
Standardized protocols for quality control of MRM-based plasma proteomic workflows. Journal of proteome research\| PubMed ID: 23245390	2013
Top-down structural analysis of posttranslationally modified proteins by Fourier transform ion cyclotron resonance-MS with hydrogen/deuterium exchange and electron capture dissociation. Proteomics\| PubMed ID: 23319428	2013
Plasma 25-Hydroxyvitamin D, Hormonal Contraceptive Use, and the Plasma Proteome in Caucasian, East Asian, and South Asian Young Adults. Journal of proteome research\| PubMed ID: 23427916	2013
Comprehensive analysis of oil sands processed water by direct-infusion Fourier-transform ion cyclotron resonance mass spectrometry with and without offline UHPLC sample prefractionation. Environmental science & technology\| PubMed ID: 23566025	2013
Metabolomic analysis of key central carbon metabolism carboxylic acids as their 3-nitrophenylhydrazones by UPLC/ESI-MS. Electrophoresis\| PubMed ID: 23580203	2013
Absolute quantitation of proteins in human blood by multiplexed multiple reaction monitoring mass spectrometry. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 23585092	2013
Multiplexed MRM-based quantitation of candidate cancer biomarker proteins in undepleted and non-enriched human plasma. Proteomics\| PubMed ID: 23592487	2013
Computational biomarker pipeline from discovery to clinical implementation: plasma proteomic biomarkers for cardiac transplantation. PLoS computational biology\| PubMed ID: 23592955	2013
Analysis of selected sugars and sugar phosphates in mouse heart tissue by reductive amination and liquid chromatography-electrospray ionization mass spectrometry. Analytical chemistry\| PubMed ID: 23682691	2013
Design, implementation and multisite evaluation of a system suitability protocol for the quantitative assessment of instrument performance in liquid chromatography-multiple reaction monitori... Molecular & cellular proteomics : MCP\| PubMed ID: 23689285	2013
Development of MRM-based assays for the absolute quantitation of plasma proteins. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 23765619	2013
Developing an iMALDI method. Methods in molecular biology (Clifton, N.J.)\| PubMed ID: 23765621	2013
Advances in multiplexed MRM-based protein biomarker quantitation toward clinical utility. Biochimica et biophysica acta\| PubMed ID: 23806606	2013
Comparison of proteins in whole blood and dried blood spot samples by LC/MS/MS. Journal of the American Society for Mass Spectrometry\| PubMed ID: 23821375	2013
Hydroxyflavones as a new family of matrices for MALDI tissue imaging. Analytical chemistry\| PubMed ID: 23895229	2013
Method and platform standardization in MRM-based quantitative plasma proteomics. Journal of proteomics\| PubMed ID: 23933160	2013
Model of the Mediator middle module based on protein cross-linking. Nucleic acids research\| PubMed ID: 23939621	2013
Metabonomics reveals drastic changes in anti-inflammatory/pro-resolving polyunsaturated fatty acids-derived lipid mediators in leprosy disease. PLoS neglected tropical diseases\| PubMed ID: 23967366	2013
HIF1A reduces acute lung injury by optimizing carbohydrate metabolism in the alveolar epithelium. PLoS biology\| PubMed ID: 24086109	2013
Metabolic Signatures of Triatomine Vectors of Trypanosoma cruzi Unveiled by Metabolomics. PloS one\| PubMed ID: 24204787	2013
Targeted quantitation of CVD-linked plasma proteins for biomarker verification and validation. Expert review of proteomics\| PubMed ID: 24206229	2013
Pre-analytical and analytical variability in absolute quantitative MRM-based plasma proteomic studies. Bioanalysis\| PubMed ID: 24256362	2013