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Intracellular Signaling Events and Hormone Secretion in Human Pseudoislets

November 3rd, 2023
*1, *1, 1, 2, 3, 2, 2, 2, 3, 1,2,4, 2
1Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 2Division of Diabetes, Endocrinology and Metabolism, Department of Medicine, Vanderbilt University Medical Center, 3Department of Biomedical Engineering, University of Miami, 4VA Tennessee Valley Healthcare System
* These authors contributed equally

Summary
Automatic Translation

Denne protokollen beskriver en metode for synkron oppkjøp og samregistrering av intracellulære signalhendelser og utskillelse av insulin og glukagon ved primære humane pseudoisleter ved bruk av adenoviral levering av en syklisk adenosinmonofosfat (cAMP) biosensor, en cAMP-differansedetektor in situ (cADDis) og et mikroperifusjonssystem.

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