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Differentiating Human Pluripotent Stem Cells into Pancreatic β-Cells

February 2nd, 2024
1, 2, 2, 1,3, 1,4,5
1Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Cell Biology and Functional Genomics, 2Departments of Pediatrics, Naomi Berrie Diabetes Center, Obstetrics and Gynecology, Columbia Stem Cell Initiative, Columbia University Irving Medical Center, Columbia University, 3Department of Diabetes, Imperial College Healthcare NHS Trust, 4Faculté de Médicine, Université de Montréal, 5Lee Kong Chian Imperial Medical School, Nanyang Technological University

Summary
Automatic Translation

Denne artikel præsenterer en protokol til rettet differentiering og funktionel analyse af β-cellelignende celler. Vi beskriver optimale dyrkningsbetingelser og passager for humane pluripotente stamceller, før der genereres insulinproducerende bugspytkirtelceller. Den seks-trins differentiering skrider frem fra endelig endodermdannelse til funktionelle β-cellelignende celler, der udskiller insulin som reaktion på glukose.

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