Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

July 28, 2023

Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues (Video) | JoVE

01:58 min

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Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing (Video) | JoVE

02:02 min

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Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

03:38 min

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Lucas Stalder, Fabian Koechl, Kerstin Hahn, Marc Sultan, Megana K. Prasad

¹Roche Pharma Research and Early Development, Pharmaceutical Sciences,Roche Innovation Centre Basel

Summary

The study describes a simple, quick, and partially automated protocol to isolate high-quality nuclei from frozen mammalian tissues for downstream single nuclei RNA sequencing.

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

Enhancing Drug Discovery - Development of Automated, Standardized Protocols for Nuclei Extraction from Frozen Tissues (Video) | JoVE

Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing (Video) | JoVE

Clean-Up and Counting of Isolated Single Nuclei from Frozen Mammalian Tissues (Video) | JoVE

Summary

Article