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Energy and Catalysis
How Fast Enzymes Work in Digestion
Description
Enzymes can work at very different speeds, and their turnover number shows how fast they convert substrate molecules into products. Turnover number is the maximum number of substrate molecules an enzyme can transform per unit time. For most enzymes, this value is between 1 and 1000 molecules per second.
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Transcript
转换数或 kcat 表示酶将底物分子转化为产物的速率有多快。
kcat 等于每个酶活性位点在单位时间内能够转化的底物分子的最大数量。
不同酶的转换数范围从每秒少于一个底物分子到每秒数百万个分子不等。
为了计算 kcat,需将酶促反应的最大反应速率(Vmax)除以总酶浓度。
催化速率常数kcat和底物亲和力常数KM均影响酶的催化效率——即酶加速特定生化反应的有效程度。
衡量特定底物催...
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