RNA催化剂作为记者为筛选药物对RNA编辑在锥虫
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Summary
Abstract
Introduction
Protocol
Representative Results
Discussion
Disclosures
The authors have nothing to disclose.
Acknowledgements
Materials
| SDM-79 Medium | Gibco by Life Technologies | ||
| Fetal Bovine Serum | Life Technologies | 12483-020 | heat inactivation at 55 °C for 1 hr |
| Hemin, minimum 80% | Sigma | H5533-10G | |
| Penicillin-Streptomycin Sollution | Fisher Scientific | MT-30-002-CI | |
| Dnase 1 recombinant, Rnase Free | Roche | 4716728001 | |
| T7 RiboMax Express Large scale RNA production system | Promega | P1320 | |
| Kimble Kontes Dounce Tissue Grinders | Fisher Scientific | K885300-0040 | |
| Gradient Master, ver 5.25 | Biocomp | 107-201M | |
| Ultra Clear Tube, 13.2 ml | Beckman Coulter | 344059 | |
| Optima L-100XP Ultracentrifuge | Beckman Coulter | 392052 | |
| SW 41 Ti ROTOR | Beckman Coulter | 331336 | |
| MicroSeal 'B' Seal, Seals | Biorad | MSB1001 | |
| CFX 384 Touch Real-Time PCR Detection System | Biorad | 185-5484 | |
| Acryl/Bis solution (19:1), 40% (w/v) | Bio Basic | A0006-500ML | |
| Urea, Molecular biology grade, 1 kg | Life Technologies | AM9902 |
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Cite This Article
Moshiri, H., Mehta, V., Salavati, R. RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes. J. Vis. Exp. (89), e51712, doi:10.3791/51712 (2014).