方法文章

Automatic Detection of Highly Organized Theta Oscillations in the Murine EEG

DOI：

10.3791/55089

⸱

2017年3月10日

 ,  ,  ,  ,  ,  ,  ,  , 
1Department of Psychiatry and Psychotherapy, University of Cologne, 2Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 3Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases, 4Federal Institute for Drugs and Medical Devices

本文内容

摘要

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海马体中的

Theta 活动与特定的认知和行为阶段有关。在这里，我们描述了一种分析方法，使用基于时间频率（即小波分析）的方法检测海马体内高度组织的 θ 振荡。

摘要

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Theta activity is generated in the septohippocampal system and can be recorded using deep intrahippocampal electrodes and implantable electroencephalography (EEG) radiotelemetry or tether system approaches. Pharmacologically, hippocampal theta is heterogeneous (see dualistic theory) and can be differentiated into type I and type II theta. These individual EEG subtypes are related to specific cognitive and behavioral states, such as arousal, exploration, learning and memory, higher integrative functions, etc. In neurodegenerative diseases such as Alzheimer's, structural and functional alterations of the septohippocampal system can result in impaired theta activity/oscillations. A standard quantitative analysis of the hippocampal EEG includes a Fast-Fourier-Transformation (FFT)-based frequency analysis. However, this procedure does not provide details about theta activity in general and highly-organized theta oscillations in particular. In order to obtain detailed information on highly-organized theta oscillations in the hippocampus, we have developed a new analytical approach. This approach allows for time- and cost-effective quantification of the duration of highly-organized theta oscillations and their frequency characteristics.

引言

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Theta activity in the brain is related to different cognitive and functional states, including arousal, attention, voluntary movement, exploratory behavior, attention behavior, learning and memory, somatosensory integration, and rapid eye movement (REM) sleep1,2. Principally, theta activity as a rhythmic entity can be generated in various cerebral regions and is highly organized and synchronized as theta oscillations. Below, we will focus on the analysis and quantification of theta activity/oscillations that are generated within the septohippocampal system3,....

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方案

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All animal experimentation was performed according to the guidelines of the local and institutional Council on Animal Care (University of Bonn, BfArM, LANUV, Germany). In addition, all animal experimentation was carried out in accordance with superior legislation, e.g., the European Communities Council Directive of 24 November 1986 (86/609/EEC), or individual regional or national legislation. Specific effort was made to minimize the number of animals used, as well as their suffering.

1. Animal Housing and EEG Recording Conditions

  1. House mice in filter-top cages or use individually-ventilated cages.
  2. Transfer mic....

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结果

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Theta activity can be recorded in a wide range of central nervous system (CNS) regions. Here, we present an analysis of theta oscillations from the murine hippocampus. Such oscillations can occur during different behavioral and cognitive states. It is highly recommended to analyze theta oscillations under both spontaneous long-term, task-related short-term, and pharmacologically-induced conditions.
Figure 1 illustrates a representative intrahippocampal CA1 recording u.......

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讨论

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Theta activity is of central relevance in systemic neurophysiology. It can be observed in various brain regions, particularly in the hippocampus, where it is related to specific behavioral and cognitive states. In addition, hippocampal theta can be pharmacologically differentiated into atropine-sensitive type II and atropine-insensitive type I theta. Type I is thought to be related to locomotion, such as walking or running27,28,29

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披露

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作者没有什么可透露的。

致谢

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作者感谢 Christina Ginkel 博士（德国神经退行性疾病中心，DZNE）和 Robert Stark 博士（DZNE）在动物育种和动物保健方面的帮助。这项工作得到了德国波恩联邦药品和医疗器械研究所 （Bundesinstitut für Arzneimittel und Medizinprodukte， BfArM） 的财政支持。

....

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卡洛芬 （Rimadyl VET - Injektionslösung）辉瑞PZN 011020820820ml
双眼手术放大显微镜Stemi 20000000001003877、4355400000000、0000001063306、4170530000000、4170959255000、4551820000000、4170959040000、4170959050000
右泛醇 （Bepanthen Wund- und Heilsalbe）拜耳PZN：1578818
窗帘（无菌）哈特曼PZN 0366787
70% 乙醇Carl Roth9065.5
0.3% / 3% 过氧化氢溶液Sigma9532130% 储备溶液
手套（无菌）Unigloves1570
牙科玻璃离聚物水泥KentDental /NORDENTA957 321
热基手术器械消毒器F.S.T.18000-50
高速牙钻AdeorSI-1708
吸入麻醉系统（异氟醚）哈佛仪器有限公司34-1352， 10-1340， 34-0422， 34-1041， 34-0401， 34-1067， 72-3044， 34-0426， 34-0387， 34-0415， 69-0230
异氟醚Baxter 250 毫升PZN 6497131
氯胺酮辉瑞PZN 07506004
乳酸林格氏液（无菌）BraunL7502
尼氏染色液Armin BaackBAA31712159
垫（无菌）ReWa Krankenhausbedarf2003/01
钢和钨电极 聚对二甲苯涂层FHC Inc.， USAUEWLGESEANND
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TA10ETA-F20DSI270-0042-001X射频发射器 3.9 g，1.9 cc，输入电压范围 & 2.5 mV，通道带宽 （B） 1 - 200 Hz，标称采样率 （f） 1,000 Hz （f = 5B） 温度工作范围 34 - 41 °C 保证电池寿命 4 个月
TL11M2-F20EETDSI270-0124-001X射频发射器 3.9 g，1.9 cc，输入电压范围 & plusmn; 1.25 mV，通道带宽 （B） 1 - 50 Hz，标称采样率 （f） 250 Hz （f = 5B） 温度工作范围 34 - 41 °C 保证电池寿命 1.5 个月
Vibroslicer 5000 MZ电子显微镜科学5000-005
甲苯噻嗪（Rompun）拜耳PZN：1320422
MatlabMathworks Inc.编程、计算和可视化软件
SPSSIBM统计分析软件
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参考文献

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  1. Vanderwolf, C. H. Hippocampal electrical activity and voluntary movement in the rat. Electroencephalogr Clin Neurophysiol. 26, 407-418 (1969).
  2. Kahana, M. J., Seelig, D., Madsen, J. R. Theta returns. Curr Opin Neurobiol. 11, 739-744 (2001).
  3. Varga, V., et al.

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