Visual Evoked Potential Recordings in Mice Using a Dry Non-invasive Multi-channel Scalp EEG Sensor

摘要

We designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable. This paper describes the whole process from manufacturing the proposed EEG electrode to signal processing of visual evoked potential (VEP) signals measured on a mouse scalp using a dry non-invasive multi-channel EEG sensor.

摘要

For scalp EEG research environments with laboratory mice, we designed a dry-type 16 channel EEG sensor which is non-invasive, deformable, and re-usable because of the plunger-spring-barrel structural facet and mechanical strengths resulting from metal materials. The whole process for acquiring the VEP responses in vivo from a mouse consists of four steps: (1) sensor assembly, (2) animal preparation, (3) VEP measurement, and (4) signal processing. This paper presents representative measurements of VEP responses from multiple mice with a submicro-voltage signal resolution and sub-hundred millisecond temporal resolution. Although the proposed method is safer and more convenient compared to other previously reported animal EEG acquiring methods, there are remaining issues including how to enhance the signal-to-noise ratio and how to apply this technique with freely moving animals. The proposed method utilizes easily available resources and shows a repetitive VEP response with a satisfactory signal quality. Therefore, this method could be utilized for longitudinal experimental studies and reliable translational research exploiting non-invasive paradigms.

引言

As the number of patients with senile degenerative brain diseases such as dementia, Alzheimer's, Parkinsonian syndromes, and stroke have increased with an aging population and an increasing life expectancy, the long-term societal burden of these diseases has also increased^1,2,3. In addition, most neurodevelopmental diseases, such as Schizophrenia and autism, are accompanied by cognitive and behavioral disorders which affect a patient's whole life^2,3,4. For this reason, resear....

方案

Animal care and handling followed the institutional guideline of the Gwangju Institute of Science and Technology (GIST).

NOTE: The procedure for acquiring the VEP signal from a mouse in vivo consists of four steps: (1) sensor assembly, (2) animal preparation, (3) VEP measurement, and (4) signal processing.

1. Sensor Assembly

1. Prepare sixteen pins for one non-invasive electrode.
   NOTE: Each pin-type electrode consists of three parts: a probe head plunger, an internal spring, and a barrel as shown in Figure 1a. The length of each pin is 13 mm, and the adjustab....

结果

We calculated the ensemble average of VEP responses from eleven mice as shown in Figure 2. This result shows the VEP responses obtained through this experiment from the pre-stimulation period (-300 ms) to the post-stimulation period (600 ms), as the stimulation is given at time 0 s. It is noticeable that the signal fluctuates only for a while (less than 300 ms) after the stimulation, while the signal steadily stabilizes over time during the post-stimulation p.......

讨论

We firstly focused on the design of the sensor, prioritizing practicality by minimizing complex surgical procedures. The deformable EEG sensor is comprised of sixteen pins: fourteen for recording, one for ground, and the last one for reference electrodes. Each electrode has the plunger-spring-barrel structure, which applies deformability onto the electrode's contact surface, so they facilitate uniform and stable signal acquisition from the curved and tender mouse's scalp. Considering the welfare of the animals, we tried .......

材料

本文使用的材料清单

姓名	公司	目录编号	评论
氯胺酮 50 注射液（小瓶）	Yuhan	-	盐酸氯胺酮 57.68 毫克
Zoletil 50 注射液	Virbac-Tiletamina		125 毫克/唑拉西泮 125 毫克
Rompun 2% 注射液。	BAYER-		盐酸甲苯噻嗪 23.32mg/mL
Hycell 溶液 2%	Samil	-	羟丙基甲基纤维素 20 mg
Puralube Vet 软膏 3.5 mg	Pharmaderm-Saline
溶液 注射液。	药明药业	-	NaCl 9 克/1000 毫升
Veet 脱毛霜 –脚 &身体 - 敏感皮肤	Reckitt Benckiser	-	脱毛
皮肤 - 手术皮肤标记	Surgmed	S-3000	STERILE - 多尖精细标记器，带尺子和标签套装
不锈钢微型刮刀	HEATHROW SCIENTIFIC	HS15907	一个圆形平头，2L x 5/16W"
棉交换
立体定位，桌面Digi单个	RWD生命科学	68025
鼠标适配器	RWD生命科学	68010
耳杆非破裂	RWD生命科学	68306
Mitsar-EEG 202-24	MITSAR		放大器
EEG研究脑电图采集软件	MITSAR
White 闪光刺激器	MITSAR	MITSAR Flash 刺激器
BCI2000 软件	Schalk 实验室
g.USBamp	g.tec	0216
g.Power-g.USBamp	g.tec	0247
441 样式直体防触摸连接器	塑料 One	441000PSW080001	441 - 000 PSW 80" （黑色）
标准探头	LEENO	SK100CSW	http://www.globalinterpark.com/detail/detail?prdNo=2114277241&dispNo=001851006012
精密雕刻机床	TINYROBO	TinyCNC-6060C
热 SHIRINK	3M	FP301