当修饰的精氨酸残基未知和/或没有特异性抗体可用时,邻位连接测定是一种非常有用的技术,用于定位和定量给定蛋白质的精氨酸甲基化。
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| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 粘附载玻片 TOMO 90°, x100 | VWR | 631-1239 | |
| 抗 GR 抗体(小鼠) | Santa Cruz | sc393232 | |
| 抗 GR 抗体(小鼠) | santa cruz | sc393232 | |
| 抗 PRMT5 抗体(兔) | Merck | 07-405 | |
| 抗 SDMA 抗体(兔) | CST | 13222 | |
| Automate d'内含 | Leica | ASP 6025 | 石蜡浸润和块制备 |
| Autostainer XL | Leica | ST5010 | Autostainer |
| Cassettes Q path macrostar III x1500 | VWR | 720-2233 | |
| CC1 Roche | 5279801001 | ||
| 柠檬酸盐缓冲液 pH 6 10x,100 mL | MMF | F/T0050 | |
| Dako 抗体稀释剂 | Dako Agilent | S202230-2 | 抗体稀释剂 |
| Discovery ChromoMap 二氨基联苯胺 (DAB) 试剂盒 | Roche | 760-159 | 二氨基联苯胺 (DAB) 试剂盒 |
| Discovery Wash | Roche | 7311079001 | |
| Duolink insitu PLA 探针抗小鼠 minus | Sigma-Aldrich | DUO92004 | PLA 试剂盒(探针抗兔 minus) |
| Duolink 原位检测试剂明场 | Sigma-Aldrich | DUO92012 | PLA 试剂盒(原位检测试剂) |
| Duolink 原位 PLA 探针 anti-rabbit plus | Sigma-Aldrich | DUO92002 | PLA 试剂盒(探针 anti-rabbit plus) |
| Duolink 原位洗涤缓冲液 明场 | Sigma-Aldrich | DUO82047 | PLA 试剂盒(原位洗涤缓冲液) |
| 乙醇 96% VOL TECHNISOLV,5 L | VWR | 83804.360 | |
| 乙醇无水 ≥99.8%, AnalaR NORMAPUR ACS, 5 L | VWR | 20821.365 | |
| EZ Prep 10x | Roche | 5279771001 | |
| Formol,即用型,5 L | MMF | F/40877-36 | 福尔马林 |
| 全自动玻璃盖玻片 | Leica CV5030 | 自动盖玻片 | |
| 玻璃盖玻片 24 x 40 | Dutscher | 100037 | |
| Hematoxylin | Ventana | 760-2021 | |
| IHC 仪器 | Roche | DISCOVERY XT | IHC |
| 自动化 LCS | Roche | 5264839001 | |
| 切片机 | Thermo Scientific | Microm HM340E | 切割组织,包括块状 |
| 封固培养基 Pertex | Histolab | 00801-FR | |
| PAP 笔,用于免疫染色 | Sigma-Aldrich | Z672548-1EA | |
| 石蜡 tek III,4 x 2,5 kg | Sakura | 4511 | |
| 巴斯德一次性移液器 | Fisher Scientific | 12583237 | |
| PBS 缓冲液 10x,100 mL | MMF | F/T0020 | |
| 反应缓冲液 10x | Roche | 5353955001 | |
| Ribo 洗涤液 10x | Roche | 5266262001 | |
| RiboCC1 | Roche | ||
| 抗小鼠二抗 | Abcam | ab133469 | |
| 二抗 OmniMap 抗兔 HRP | Roche | 760-4311 | |
| 组织包埋中心 | MMF | EC 350 | |
| 二甲苯(异构体混合物)≥98.5%,AnalaR NORMAPUR ACS,5 L | VWR | 28975.360 | |
| 蔡司 Axio Imager M2 显微镜 | 正置明场显微镜 |
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