Enhanced Ascorbate Peroxidase 2
Enhanced Ascorbate Peroxidase 2 - Related Videos
11:56
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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Cited by 4 •
2014
Ascorbate plays numerous important roles in cellular metabolism, many of which have only come to light in recent years. Here we describe a medium-throughput, specific and inexpensive microplate assay for the determination of both intra- and extracellular ascorbate in cell culture.
05:17
Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples
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Cited by 2 •
2016
This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.
04:36
The Examination of Peroxidase-Positive Leukocytes in Semen
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Cited by 3 •
2024
This paper presents an economical and efficient protocol for examining peroxidase-positive leukocytes in semen. With the assistance of a computer-assisted semen analysis (CASA) system, the concentration of peroxidase-positive leukocytes in semen can be obtained within a total of 60 min, effectively improving the efficiency of andrology laboratory and andrologists.
03:00
Self-Evaluation: Self-Enhancement and Self-Verification
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Social psychologists have documented that feeling good about ourselves and maintaining positive self-esteem is a powerful motivator of human behavior (Tavris & Aronson, 2008). In the United States, members of the predominant culture typically think very highly of themselves and view themselves as good people who are above average on many desirable traits (Ehrlinger, Gilovich, & Ross, 2005). Often, our behavior, attitudes, and beliefs are affected when we experience a threat to our...
05:50
Contrast Enhanced Vessel Imaging using MicroCT
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Cited by 17 •
2011
Contrast enhanced small animal vessel imaging by microCT is a rapid, cost-effective and high-throughput technique for serial in situ examination for tumor development, for analyzing the network of blood vessels that nourish them, and for following the response of tumors to preclinical therapeutic intervention(s).
FAQs
Hydrogen peroxide activates the heme peroxidase, allowing Enhanced Ascorbate Peroxidase 2 to oxidize biotin-phenol into reactive radicals. These short-lived species covalently attach biotin to nearby proteins before diffusing far from the selected location. The resulting labeling chemistry connects molecular identity with local cellular position, which is essential for analyzing spatially organized biochemical systems.
Short-lived radicals restrict labeling to molecules near the enzyme during the activation period. This limited reaction range helps retain information about the selected protein or cellular compartment instead of producing a broadly distributed signal. Consequently, the method can resolve local molecular environments and support studies of organelle organization or changing protein neighborhoods in living cells.
Enhanced Ascorbate Peroxidase 2 offers rapid labeling kinetics and improved activity, enabling molecular environments to be captured in living cells. Conventional fixation or purification can be difficult to use for processes that change quickly or depend on cellular organization. By tagging nearby molecules before those relationships are lost, this approach supports higher-resolution analysis of dynamic biochemical events.
Researchers first position Enhanced Ascorbate Peroxidase 2 at a selected protein or cellular compartment, then provide biotin-phenol and hydrogen peroxide to initiate local labeling. Biotinylated molecules are subsequently enriched and analyzed by mass spectrometry or imaging. This sequence converts a transient cellular neighborhood into an experimentally measurable molecular profile.
Enriched biotinylated molecules can be analyzed to map subcellular proteomes, examine protein interactions, and characterize organelle organization. Mass spectrometry provides molecular identification across the labeled population, whereas imaging preserves visual information about where labeled molecules occur. The selected readout therefore determines whether the experiment emphasizes molecular composition, spatial distribution, or both.
Its rapid kinetics allow researchers to record molecular neighborhoods over a short labeling period while cells remain living. This is valuable when protein associations or organelle organization change too quickly for conventional fixation or purification methods to represent accurately. In biochemistry, the technique therefore links transient cellular events with measurable changes in local protein composition.