Leukocyte Lipid Profile
Leukocyte Lipid Profile - Related Videos
08:54
NMR Spectroscopy as a Robust Tool for the Rapid Evaluation of the Lipid Profile of Fish Oil Supplements
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Cited by 21 •
2017
Here, high-resolution 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy was used as a rapid and reliable tool for quantitative and qualitative analysis of encapsulated fish oil supplements.
05:12
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
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Cited by 11 •
2015
This simplified application of intravital microscopy of mesentery veins in mice can be used in different models of inflammation to observe leukocyte-endothelial and platelet-leukocyte interactions.
06:44
Isolation of Brain-infiltrating Leukocytes
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Cited by 33 •
2011
A rapid method to obtain infiltrating leukocytes from the murine brain is described. This method utilizes a continuous Percoll gradient and discontinuous Ficoll gradient to select and purify the leukocyte-enriched layer. Isolated leukocytes may then be characterized by flow cytometric measurements.
09:18
Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
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Cited by 11 •
2013
Mammalian integument contains solvent-extractable lipids that can provide chemical compositions characteristic of individual species. This paper presents a routine method for separating broad lipid classes isolated from integumentary tissues using thin layer chromatography and determining the triacylglyceride profile by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
06:53
Selective Harvesting of Marginating-hepatic Leukocytes
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Cited by 1 •
2016
Marginating-hepatic leukocytes exhibit unique characteristics and distinct immunological functions compared to other leukocyte populations. Here we describe a method for selective harvesting of this specific hepatic cell population, through forced perfusion of the liver of rats or mice. Marginating-hepatic leukocytes seem critical in determining susceptibility to hepatic-related diseases and metastases.
FAQs
Immune activation can remodel lipid synthesis, storage, and turnover in leukocytes. Changes in these processes may alter the relative abundance of lipids associated with membrane structure, energy storage, and signaling, linking cellular metabolism with inflammatory behavior. Measuring the profile therefore provides biochemical evidence of how immune-state changes are reflected in leukocyte composition.
Comparing phospholipids, cholesterol, triglycerides, and signaling lipids shows how different biochemical functions are represented within leukocytes. Relative abundance is important because it describes the balance among lipid classes, not merely their presence. This comparison can help connect membrane-related properties, energy storage, and signaling capacity with broader patterns of cellular function.
Relative abundance helps distinguish whether a change reflects one lipid class or a broader redistribution across the profile. That distinction is useful when relating leukocyte metabolism to inflammation, because immune responses may affect synthesis, storage, and turnover in different ways. The resulting pattern can provide a more informative biochemical view than examining an isolated lipid measurement.
A typical workflow begins by isolating leukocytes and extracting their lipids. The extracted material is then separated into classes such as phospholipids, cholesterol, triglycerides, and signaling lipids, followed by quantification. This sequence preserves the connection between the cellular sample and its biochemical measurements, allowing researchers to evaluate lipid composition in relation to leukocyte function.
Chromatographic or mass spectrometric methods can be used to analyze the extracted lipids. These approaches support separation and quantification of major classes, including phospholipids, cholesterol, triglycerides, and signaling lipids. Applying an analytical method after leukocyte isolation and lipid extraction produces measurements that can be interpreted as a profile of cellular lipid composition.
Researchers use this approach when they need to connect immune-cell metabolism with inflammation, metabolic disorders, or inflammatory disease. It can also support investigations of potential lipid-based biomarkers that distinguish physiological from pathological states. In biochemistry, the method provides a way to examine cellular function through measurable changes in leukocyte lipid composition.
The analysis can reveal patterns in lipid composition and relative abundance that relate to membrane structure, energy storage, signaling, and cellular function. When compared across physiological or pathological states, these patterns may help associate leukocyte metabolism with immune responses or disease-related inflammation. Such associations also support evaluation of lipids as potential biochemical biomarkers.