Octanol-water Partition
Octanol-water Partition - Related Videos
09:45
On-Chip Octanol-Assisted Liposome Assembly for Bioengineering
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Cited by 9 •
2023
The present protocol describes octanol-assisted liposome assembly (OLA), a microfluidic technique to generate biocompatible liposomes. OLA produces monodispersed, micron-sized liposomes with efficient encapsulation, allowing immediate on-chip experimentation. This protocol is anticipated to be particularly suitable for synthetic biology and synthetic cell research.
01:14
Extraction: Partition and Distribution Coefficients
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2024
The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium. For extracting a solute from an aqueous phase into an organic...
10:59
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
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Cited by 9 •
2012
A technique to probe the lipid raft partitioning of fluorescent proteins at the plasma membrane of living cells is described. It takes advantage of the disparity in diffusion times of proteins located inside or outside of lipid rafts. Acquisition can be performed dynamically in control conditions or after drug addition.
08:17
Water Quality Analysis via Indicator Organisms
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba -The University of Arizona Demonstrating Author: Luisa Ikner Water quality analysis monitors anthropogenic influences such as pollutants, nutrients, pathogens, and any other constituent that can impact the water’s integrity as a resource. Fecal contamination contributes microbial pathogens that threaten plant, animal, and human health with disease or illness. Increasing water demands and strict quality standards require that water...
11:11
Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
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Cited by 5 •
2018
Here, we present a protocol to perform a quantitative analysis of the level of plasma-membrane association for fluorescently-tagged peripherally-associated protein. The method is based on the computational decomposition of membrane and cytoplasmic component of signal observed in cells labeled with plasma membrane fluorescent marker.
FAQs
Because logP refers to the un-ionized form, it provides a focused estimate of how that chemical form favors octanol over water. An ionizable compound may require logD instead, because its distribution depends on pH and reflects behavior under that condition. This distinction prevents a single hydrophobicity value from being applied indiscriminately.
At equilibrium, the relevant measurement is the concentration ratio between the octanol and water phases, rather than the amount initially added to either phase. Expressing that ratio as logP makes differences in relative hydrophobicity easier to compare across compounds. The resulting value is therefore an equilibrium property, not simply a description of mixing during handling.
Octanol-water partition data connects molecular distribution to several bioengineering-relevant behaviors. A compound with greater preference for the organic phase may show different expectations for membrane permeation, aqueous solubility, biodistribution, or release from a biomaterial than one with lower preference. These relationships make partition measurements useful as predictive inputs during early molecule and formulation assessment.
The essential workflow is to evaluate the compound after it has reached equilibrium between octanol and water, then compare its concentrations in the two phases. Reporting the un-ionized concentration ratio as logP, or the pH-dependent distribution as logD for ionizable substances, produces a value suitable for downstream interpretation in bioengineering studies.
During pharmaceutical screening, partition data helps prioritize molecules by indicating expected relationships among hydrophobicity, solubility, and membrane permeation. It also contributes to biodistribution assessment, which supports early comparison of biologically active compounds. This measurement does not replace biological testing, but it provides a physicochemical basis for selecting candidates for further evaluation.
In delivery-system design, partition data helps connect a molecule's phase preference with its anticipated release from biomaterials. Bioengineers can consider it alongside solubility and membrane permeation when comparing active molecules or delivery systems before further development. Its role is predictive: the measurement informs design decisions rather than directly measuring drug release itself.