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Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
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JoVE Journal Biology
Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

DOI:
10.3791/57837-v

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11:11 min

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June 15, 2018

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, ,
1Department of Experimental Plant Biology,Faculty of Science, Charles University

Chapters

  • 00:04Title
  • 00:47Confocal Laser Scanning Microscopy
  • 01:49Required Software Installation
  • 02:45Image Analysis in Fiji
  • 09:27Results: Quantification of Plasma Membrane Partitioning for Examined Samples
  • 10:33Conclusion

Summary

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Here, we present a protocol to perform a quantitative analysis of the level of plasma-membrane association for fluorescently-tagged peripherally-associated protein. The method is based on the computational decomposition of membrane and cytoplasmic component of signal observed in cells labeled with plasma membrane fluorescent marker.

Tags

Plasma Membrane PartitioningPeripherally-associated ProteinsCell BiologyCell SignalingMicroscopyFM 4-64 DyeConfocal ImagingFiji ImageJR ProjectImage Analysis

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