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Biology

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Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins
 

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

Article doi: 10.3791/57837
June 15th, 2018

Summary June 15th, 2018

Here, we present a protocol to perform a quantitative analysis of the level of plasma-membrane association for fluorescently-tagged peripherally-associated protein. The method is based on the computational decomposition of membrane and cytoplasmic component of signal observed in cells labeled with plasma membrane fluorescent marker.

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