Perivascular Cell Recruitment
Perivascular Cell Recruitment - Related Videos
07:05
Use of Human Perivascular Stem Cells for Bone Regeneration
0 Views •
Cited by 42 •
2012
Human perivascular stem cells (PSCs) are a novel stem cell class for skeletal tissue regeneration similar to mesenchymal stem cells (MSCs). PSCs can be isolated by FACS (fluorescence activated cell sorting) from adipose tissue procured during standard liposuction procedures, then combined with an osteoinductive scaffold to achieve bone formation in vivo.
08:15
Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue
0 Views •
Cited by 7 •
2016
Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium.
09:05
A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
0 Views •
Cited by 4 •
2017
Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.
04:28
Lung Tumor Cell Recruitment Assay
0 Views •
2019
This manuscript describes a method to quantify tumor cell accumulation in the lungs in an animal model of tumor metastasis.
05:52
An In Vitro Flow Investigation of the Effect of Stromal Cells on Leukocyte Recruitment
0 Views •
2025
The video demonstrates a method for investigating the impact of stromal cells on leukocyte recruitment. In a parallel plate flow chamber model, pro-inflammatory cytokines induce the expression of adhesion receptors by endothelial cells, facilitating neutrophil recruitment and migration. The cross-talk between stromal cells and endothelial cells diminishes neutrophil recruitment to the endothelial cells.
FAQs
Recruitment is coordinated through signals released by endothelial cells, injured tissue, or biomaterials. These cues direct perivascular cells toward developing vessels rather than leaving their movement unregulated. After reaching the vessel, the cells adhere to its surface, positioning them to provide structural and regulatory support. This signaling-to-adhesion sequence is central to vascular stabilization.
Pericytes and vascular smooth muscle cells contribute more than physical coverage. Once associated with a vessel, they provide extracellular matrix and regulatory signals that help organize the vessel environment. Their recruitment therefore links cellular positioning with the material and communication requirements of vascular stability. In engineered systems, considering both cell types can help reproduce support functions needed by developing microvascular networks.
Recruitment cues may arise from three settings identified in the source material: endothelial cells, injured tissue, and biomaterials. This makes the process responsive to both biological conditions and engineered environments. For bioengineers, the relevant design question is whether a developing vessel or scaffold presents signals capable of attracting support cells and enabling their subsequent association with the vessel.
An effective recruitment sequence has three linked stages: directed migration toward a developing vessel, adhesion at the vessel surface, and delivery of extracellular matrix and regulatory signals. Each stage contributes a different function, so observing movement alone would not establish complete support-cell association. In bioengineering studies, this sequence provides a framework for assessing whether vascular development progresses toward stability.
Successful control of perivascular cell recruitment can support stable, functional microvascular networks in engineered tissues, organoids, and regenerative scaffolds. The expected relevance extends beyond vessel formation: appropriate association may improve vascular integration and tissue perfusion. These outcomes make recruitment especially relevant when the goal is to improve how vascular structures function within engineered or regenerative constructs.
Perivascular cell recruitment connects cell behavior with the design of regenerative environments. Researchers can consider it when developing engineered tissues, organoids, or scaffolds intended to support vascular repair and regeneration. The same mechanistic understanding also informs therapies for tissue repair, where vascular stability, integration, and perfusion are important desired outcomes.