Staple Crossovers
Staple Crossovers - Related Videos
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The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage
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The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.
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Crossover Experiments
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Crossover experiments, also called the repeated-measurements design, is a study design in which all experimental units are exposed to all treatments in different periods. Crossover experiments are generally used in psychology, the pharmaceutical industry, agriculture, and medicine. Crossover designs are performed even with smaller sample sizes since the samples can act as their controls. These are better than simple randomized trials since patients are exposed to all the treatments.
02:27
Generating Double-Crossover Recombinants of Pseudomonas aeruginosa for Targeted Gene Deletion
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Source: Valentine, M. E., et al. Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection. J. Vis. Exp. (2020).This video demonstrates the generation of double-crossover recombinants in Pseudomonas aeruginosa using a plasmid-based gene deletion strategy. The process involves selecting bacteria that harbor a non-replicative plasmid integrated into the genome via a single crossover and then inducing a second...
06:18
Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR
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2025
This protocol provides a method for measuring crossover frequencies and distribution during meiosis in Caenorhabditis elegans. Single-nucleotide polymorphism genotyping with real-time PCR was used to determine the origin of the chromosomes. The 4-point mapping allowed to determine the crossover distributions of the left-arm, center, and right-arm regions of each chromosome. ...
13:32
Designing a Bio-responsive Robot from DNA Origami
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Cited by 3 •
2013
DNA origami is a powerful method for fabricating precise nanoscale objects by programming the self-assembly of DNA molecules. Here, we describe how DNA origami can be utilized to design a robotic robot capable of sensing biological cues and responding by shape shifting, subsequently relayed to a desired effect.
FAQs
Crossover placement acts as a geometric control point: it determines where a staple connects neighboring scaffold helices and therefore helps set their relative alignment. Because the same placement also contributes to how adjacent duplexes are held together, designers use planned positions to balance the intended three-dimensional shape with mechanical integrity during folding.
The key interaction is complementary binding across two scaffold-helix segments. A staple recognizes each matching segment, then switches between neighboring helices at the planned crossover site. This links local sequence recognition to larger-scale organization: accurate pairing and switching help align adjacent duplexes and support formation of the intended DNA nanostructure.
Spacing matters because crossover locations collectively define how the helices are organized rather than acting as isolated connections. Adjusting the distance between planned sites can improve folding accuracy and preserve the intended geometry. This design variable is especially important when a structure must maintain a reliable three-dimensional framework for later functional patterning.
A design workflow begins with the desired three-dimensional framework, followed by selection of complementary staple segments on neighboring scaffold helices and placement of crossover sites. Designers then consider how that pattern affects alignment, geometry, and mechanical integrity. This connects sequence-level design decisions with the final nanoscale architecture.
Staple crossovers support addressable nanoscale frameworks in several formats. The overview identifies molecular cages, patterned surfaces, and assemblies for biosensing or targeted delivery as applications. Their value lies in organizing structural features and functional molecules at defined locations, allowing bioengineers to build DNA-based systems whose architecture is linked to a specific use.
In bioengineering, these connections provide a route from programmable DNA folding to functional materials and tools. By controlling crossover spacing and placement, researchers can improve folding accuracy while arranging functional molecules within a designed framework. The resulting structures can support biosensing, targeted delivery, or other DNA-based applications where nanoscale organization is important.