Ultrasonic Bath Dispersion

Ultrasonic bath dispersion is a sample-processing technique that uses sound energy to distribute particles, cells, or other materials uniformly throughout a liquid. In a bath, high-frequency pressure waves generate cavitation bubbles whose formation and collapse produce localized fluid motion, shear, and mixing, helping break up agglomerates without requiring direct probe contact. Bioengineers use this approach to prepare nanoparticle suspensions, homogenize biomaterial formulations, and improve sample consistency for fabrication or analysis. Dispersion conditions such as frequency, power, treatment time, temperature, and liquid volume influence particle size, stability, and biological integrity, making controlled optimization essential for reproducible results.

Ultrasonic Bath Dispersion - Related Videos

Research

JoVE Journal - Environment

Functionalization and Dispersion of Carbon Nanomaterials Using an Environmentally Friendly Ultrasonicated Ozonolysis Process

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Cited by 12 •

2017

Here, a novel method for the functionalization and stable dispersion of carbon nanomaterials in aqueous environments is described. Ozone is injected directly into an aqueous dispersion of carbon nanomaterial that is continuously recirculated through a high-powered ultrasonic cell.

Research

JoVE Journal - Environment
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Dispersion of Nanomaterials in Aqueous Media: Towards Protocol Optimization

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Cited by 73 •

2017

Here, we present a step-wise protocol for the dispersion of nanomaterials in aqueous media with real-time characterization to identify the optimal sonication conditions, intensity, and duration for improved stability and uniformity of nanoparticle dispersions without impacting the sample integrity.

Research

JoVE Journal - Medicine
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Formation of Dispersible Taohong Siwu Tablets

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2023

This article discusses the process used to formulate and prepare Taohong Siwu, a traditional Chinese medicine, as a dispersible tablet. We performed single-factor and orthogonal experiments to screen the prescription excipients, optimize the formulation process, and produce dispersible Taohong Siwu tablets.

Ultrasonic-Assisted Extraction of Cannabidiolic Acid from Cannabis Biomass

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Cited by 5 •

2022

Ultrasonic-assisted extraction (UAE) increases extraction efficiency of solvents and when applied to Cannabis spp. biomass it reduces the time required for extraction. This decreases the cost and potential cannabinoid loss due to degradation. Additionally, UAE is considered a green method due to low solvent use.

Ultrasonic-augmented Primary Adult Fibroblast Isolation

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Cited by 9 •

2019

We present a protocol to isolate primary adult fibroblasts in an easy, fast and reliable way, performable by beginners (e.g., students). The procedure combines enzymatic tissue digestion and mechanical agitation with ultrasonic waves to obtain primary fibroblasts. The protocol can easily be adapted to specific experimental requirements (e.g., human tissue).

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