Fluorescence Microendoscopy Imaging
Fluorescence Microendoscopy Imaging - Related Videos
13:49
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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Cited by 84 •
2011
In many biological and clinical situations it is advantageous to study cellular processes as they evolve in their native microenvironment. Here we describe the assembly and use of a low-cost fiber-optic microscope which can provide real time imaging in cell culture, animal studies, and clinical patient studies.
10:35
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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2016
The assembly and use of a multimodal microendoscope is described which can co-register superficial tissue image data with tissue physiological parameters including hemoglobin concentration, melanin concentration, and oxygen saturation. This technique can be useful for evaluating tissue structure and perfusion, and can be optimized for individual needs of the investigator.
14:10
Video-rate Scanning Confocal Microscopy and Microendoscopy
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Cited by 14 •
2011
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
07:12
Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy
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Cited by 7 •
2021
We present a protocol for the use of fiberoptic confocal laser microendoscopy (CLM) to non-invasively study the spatio-temporal distribution of liposomes in the eye after subconjunctival injection.
FAQs
The system directs excitation light to a labeled target and collects the fluorescence emitted afterward. Spectral filtering separates this emitted signal from the incoming excitation light before conversion into an image. This sequence allows researchers to visualize labeled cells, molecules, or tissues with molecular specificity while preserving spatial information about their location within a living specimen.
Fluorescent labels provide the molecular contrast that makes selected cells, molecules, or tissues visible against surrounding structures. Because the detected emission is associated with the labeled target, the resulting image can reveal more than general tissue appearance. This specificity supports evaluation of cellular responses and interactions between engineered materials and biological systems.
Compact lenses and fiber-optic probes deliver excitation light and collect emitted fluorescence close to the region being examined. Their small form supports access to living specimens while reducing the need to remove large amounts of tissue or material for observation. In bioengineering, this makes repeated, in situ assessment of tissue constructs and biomaterial interactions more practical.
By producing images that contain both spatial and temporal information, the technique can show where labeled features occur and how they change during observation. Real-time monitoring is especially relevant when researchers need to follow cellular responses, tissue behavior, or biomaterial interactions within living engineered systems rather than relying only on endpoint examination.
A typical workflow begins by introducing or identifying fluorescently labeled cells, molecules, or tissues in the specimen. Excitation light is delivered through a compact lens or fiber-optic probe, and the resulting emission is collected. Spectral filtering then isolates the fluorescence, which is converted into images for spatial or temporal assessment of the target.
Researchers may choose this approach when they need minimally invasive, real-time observation within a living specimen. It is suited to studies of tissue constructs, biomaterial interactions, disease models, and cellular responses. The ability to monitor these systems in situ can reduce reliance on extensive sample removal and provide information during, rather than only after, an experiment.
The method supplies in situ visual information about engineered systems and biological responses, helping researchers assess how cells, tissues, and biomaterials behave under observation. Those findings can guide refinement of diagnostic and therapeutic technologies. Its combination of molecular specificity, compact instrumentation, and real-time monitoring connects biological evaluation with the iterative design of bioengineering solutions.