Autoclavable Glass Bioreactor
Autoclavable Glass Bioreactor - Related Videos
06:34
Proper Use of Autoclaves
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2023
Robert M. Rioux & Zhifeng Chen, Pennsylvania State University, University Park, PA Autoclaving is one of the most commonly used methods in the laboratory for the purpose of decontamination. The most common items decontaminated with an autoclave are those that contact biological samples (typically those containing microorganisms). An autoclave works by utilizing pressurized high temperature steam to kill microorganisms present in the loaded materials. Sufficient steam flow and heat transfer...
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
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Cited by 28 •
2016
The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments16-21. This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.
11:22
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Cited by 32 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
FAQs
Controlled cultivation depends on coordinating several independently regulated conditions. Agitation and aeration support the cultivation environment, while temperature, pH, and dissolved oxygen provide additional control points. Adjusting these variables allows investigators to examine cell growth, metabolism, and product formation under defined laboratory conditions, making comparisons between experiments more reproducible.
Glass provides direct visual access to the culture and vessel interior, so researchers can observe the system during an experiment rather than relying only on regulated parameters. Its ability to tolerate repeated sterilization also supports reuse between experiments. Together, these features aid monitoring and contribute to consistent laboratory workflows.
Sterilizing the assembled vessel and its compatible components with pressurized steam prepares the system for work under controlled laboratory conditions. Treating the complete compatible setup, rather than only the glass vessel, helps establish a uniform starting point for cultivation. This preparation is especially important when experiments must be repeated and compared.
Researchers first assemble the vessel with components that are compatible with autoclaving, then expose the assembled system to pressurized steam in an autoclave. After sterilization, they conduct cultivation while regulating selected process conditions, such as agitation, aeration, temperature, pH, and dissolved oxygen. This sequence links preparation with controlled biological experimentation.
It is useful when a study requires controlled cultivation of microorganisms, cells, or tissues and benefits from observing the vessel during the run. Biology and biotechnology researchers can apply it to investigations of cell growth, metabolism, and product formation, as well as process development and small-scale production. Reusability also supports repeated laboratory studies.
By regulating cultivation conditions and observing the transparent vessel, researchers can generate information about how biological systems grow and function under defined laboratory settings. The resulting studies can address cell growth, metabolism, and product formation. Because the vessel can be sterilized between experiments, researchers can use the system for reproducible comparisons across repeated studies.