Journal
/
Bioengineering
/
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

DOI:
10.3791/2646-v

•

11:22 min

•

June 14, 2011

•

,
1Department of Biomedical Engineering,Yale University, 2Department of Anesthesiology,Yale University School of Medicine

Chapters

  • 00:05Title
  • 01:46Preparation of Polyglycolic Acid (PGA) Scaffold
  • 02:55Assembly of Bioreactor
  • 04:33Bioreactor Setup and Seeding Cells
  • 07:55Turning on the Pump, and First Feeding
  • 09:17Staining of Engineered Smooth Muscle Vessels
  • 10:11Conclusion

Summary

Automatic Translation

Automatic Translation

Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.

Tags

Biological-based Vascular GraftsPulsatile BioreactorArterial Bypass RegenerationVessel RegenerationBioreactor Culture SystemsSmooth Muscle Cells (SMCs)Polyglycolic Acid (PGA) MeshSilicone TubingPulsatile StimulationChemo-mechanical EnvironmentNative VesselsEndothelial Cells (EC)Animal Implantation Models

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2646?article_type=v

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering
check_url/2646?article_type=v

Operation of a Benchtop Bioreactor
check_url/2646?article_type=v

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
check_url/2646?article_type=v

Construction and Characterization of a Novel Vocal Fold Bioreactor
check_url/2646?article_type=v

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
check_url/2646?article_type=v

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
check_url/2646?article_type=v

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
check_url/2646?article_type=v

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
check_url/2646?article_type=v

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
check_url/2646?article_type=v

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Read Article