Barley Infection

Barley infection is the invasion and colonization of barley plants by pathogens, including fungi, bacteria, and viruses, that can disrupt growth and reduce yield. Infection typically begins when a pathogen reaches a susceptible plant surface, where environmental conditions support attachment, germination or entry, followed by multiplication and movement through host tissues; barley responds through physical barriers and immune defenses. Studying these interactions helps researchers identify disease symptoms, trace infection routes, and evaluate host resistance. This knowledge supports crop protection, breeding of disease-resistant barley varieties, and management strategies that improve agricultural productivity and grain quality.

Barley Infection - Related Videos

Research

JoVE Journal - Developmental Biology

Visualizing Early Infection Sites of Rice Blast Disease (Magnaporthe oryzae) on Barley (Hordeum vulgare) Using a Basic Microscope and a Smartphone

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2023

This is a straightforward protocol of a barley leaf sheath assay using minimal reagents and common laboratory equipment (including a basic smartphone). The purpose is to visualize the early infection process of blast disease in labs without access to advanced microscopy equipment.

Metabolomic Analysis of Barley by Gas Chromatography/Mass Spectrometry

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Cited by 1 •

2024

A method for metabolomic analysis of barley is presented. The method entails fractionation and derivatization of metabolites and analysis thereof by gas chromatography/mass spectrometry (GC/MS). Metabolomic analysis can be used to determine the effect of intercropping and drought stress on the grain.

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

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Cited by 1 •

2018

An improved protocol is presented for the measurement of transient gene expression from reporter constructs in barley aleurone cells after particle bombardment. The combination of automated grain grinding with 96-well plate enzyme assays provides high throughput for the procedure.

Research

JoVE Journal - Immunology and Infection
Free Sample

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection

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Cited by 108 •

2013

The larva of the wax moth Galleria mellonella was recently established as an in vivo model to study Legionella pneumophila infection. Here, we demonstrate fundamental techniques to characterize the pathogenesis of Legionella in the larvae, including inoculation, measurement of bacterial virulence and replication as well as extraction and analysis of infected hemocytes.

Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis

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Cited by 3 •

2016

This manuscript describes the use of a bioluminescent strain of African trypanosomes to enable the tracking of late stage infection and demonstrates how in vivo live imaging can be used to visualize infections within the central nervous system in real-time.

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