Basal Bodies
Basal Bodies - Related Videos
00:55
Laminins are the Adhesive Proteins of Basal Lamina
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2023
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains. In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
07:59
Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads
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Cited by 10 •
2017
This protocol describes the method of determining basal and forskolin-stimulated lipolysis in inguinal fat pads obtained from normal chow diet (NCD) or high fat diet (HFD) ± capsaicin fed wild type mice. As an index for lipolysis, glycerol release was measured from inguinal adipose fat pads.
01:05
Type IV Collagen of Basal Lamina
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2023
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions. A type IV collagen molecule has six alpha chains which can exist in...
01:03
Basal Lamina are the Specialized Form of ECM
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2023
The basal lamina is a thin extracellular layer that lies underneath the cells and separates them from other tissues. The three layers of the basal lamina are lamina lucida, lamina densa and lamina reticularis. The basal lamina, a mixture of glycoproteins and collagen, provides an attachment site for the epithelium, separating it from underlying connective tissue. The framework of basal lamina has other essential proteins such as laminins mesh, perlecan, entactin, and type IV collagen. Proteins...
11:52
Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea
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Cited by 17 •
2012
Here we demonstrate our protocol for isolation of basal and submucosal gland duct cells from mouse tracheas. We also demonstrate the method of injecting stem cells into the dorsal mouse fat pad to create an in vivo model of submucosal gland regeneration.
FAQs
The stable microtubule triplets provide an organized foundation from which axonemal microtubules extend into the developing cilium or flagellum. This arrangement links the basal body to the internal structure required for ciliary or flagellar function. Examining triplet organization therefore helps researchers understand how these surface projections acquire their architecture.
Position and attachment determine where a cilium or flagellum emerges at the cell surface. Associated proteins regulate these properties, along with basal body maturation, helping coordinate the projection with overall cell polarity. Disruption of these controls can therefore affect both the placement of cilia and the organization of the cell surface.
A basal body provides the structural starting point for axonemal microtubules, but the resulting projection can serve different roles. Motile cilia and flagella support movement, whereas sensory cilia contribute to cellular sensing. This shared organizing system allows related structures to support distinct biological functions in protists, humans, and other eukaryotes.
Investigating assembly can identify how centrioles become mature basal bodies and how associated proteins control their position and attachment. These observations connect structural changes with the formation of cilia and flagella. As a result, assembly studies provide a way to explain how cells establish polarity and produce functional surface projections.
Defects in basal body formation, maturation, positioning, or attachment can interfere with cilia and flagella. Because these structures support motility, sensing, and other cellular functions, abnormalities may contribute to developmental disorders, impaired mucociliary clearance, and infertility. Studying the defect at the basal body level helps connect cellular organization with these outcomes.
Basal bodies occur in systems ranging from protists to humans and support motile, sensory, and flagellar structures. Comparing them across organisms can clarify shared principles of microtubule organization, maturation, and surface attachment. This broad relevance makes basal body research valuable for linking basic cell biology with disorders caused by abnormal cilia.