Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

Journal

Biology

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

DOI:

10.3791/3731-v

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11:52 min

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September 14, 2012

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Ahmed E. Hegab, Vi Luan Ha, Yasser S. Attiga, Derek W. Nickerson, Brigitte N. Gomperts

¹Department of Pediatrics,David Geffen School of Medicine at UCLA

Chapters

00:05Title
01:45Dissection of Trachea
02:46Cleaning and Cutting the Trachea
03:45Enzyme Digestion and Processing into Single Cell Suspension
06:42Staining for FACS and Sorting
07:23Processing Sorted Cells for in vitro Culture and in vivo Model
09:40Representative Data
11:19Conclusion

Summary

Automatic Translation

Here we demonstrate our protocol for isolation of basal and submucosal gland duct cells from mouse tracheas. We also demonstrate the method of injecting stem cells into the dorsal mouse fat pad to create an in vivo model of submucosal gland regeneration.

Isolation of Basal Cells and Submucosal Gland Duct Cells from Mouse Trachea

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