Blood Sample Handling
Blood Sample Handling - Related Videos
01:02
Sample Handling
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2024
Transportation of samples from the collection point to the laboratory, as well as storage and preservation techniques, are crucial for maintaining sample integrity and ensuring accurate and reliable test results. Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...
10:47
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
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Cited by 13 •
2013
TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
01:37
Habituation and Positive Reinforcement for Rodent Handling
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Habituation and positive reinforcement are refinement strategies that help reduce stress during routine handling and experimental procedures in rodents. These approaches improve animal welfare, encourage voluntary cooperation,...
15:57
Rodent Handling and Restraint Techniques
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2015
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN It has been demonstrated that even minimal handling of mice and rats is stressful to the animals. Handling for cage changing and other noninvasive procedures causes an increase in heart rate, blood pressure, and other physiological parameters, such as serum corticosterone levels. Fluctuations can continue for up to several hours. The methods of restraint required for injections and blood...
FAQs
Anticoagulants help prevent clotting when the analysis requires blood cells or plasma to remain available in the specimen. Their use must match the intended analysis and container because an unsuitable choice can alter the sample’s cellular or molecular properties. Selecting the appropriate anticoagulant therefore supports accurate measurements and improves consistency between biological experiments.
Time and temperature influence how well a blood specimen preserves its cellular and molecular properties after collection. Poor control can promote clotting, hemolysis, or degradation, creating preanalytical variation before testing begins. Consistent transport and storage conditions help limit these changes, making biological results more reliable and improving comparisons across experiments or laboratories.
Centrifugation separates blood cells from the liquid portion of a specimen, allowing researchers to obtain plasma or serum for downstream analysis. This separation creates a more suitable fraction for examining particular biological properties while keeping cellular material distinct. The resulting fractions can support hematology, clinical diagnostics, immunology, genetics, and cell-based research when handled consistently.
A reliable workflow begins with controlled collection and immediate, accurate labeling. The specimen is placed in a suitable container, transported under controlled conditions, and processed as needed, including centrifugation when separation is required. Appropriate storage follows processing. Coordinating these steps reduces preanalytical variation and helps ensure that the analyzed material still represents the original biological sample.
Researchers should distinguish between serum and plasma according to the biological analysis they plan to perform. Blood handling uses anticoagulants when plasma or un-clotted cellular material is needed, whereas centrifugation can separate serum or plasma from blood cells. Making this choice before collection helps align the container and processing sequence with the intended measurement.
Careful handling improves the reliability and reproducibility of results by limiting changes that occur before analysis. Accurate labeling preserves the connection between a specimen and its source, while controlled transport, processing, and storage reduce unwanted variation. These practices are important across hematology, diagnostics, immunology, genetics, and cell-based research, where consistent samples support more dependable interpretation.