Cell Lysis
Cell Lysis - Related Videos
01:32
Tissue Homogenization and Cell Lysis
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2023
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods. Mechanical methods of tissue homogenization These methods rely on applying external physical force to disrupt...
09:49
A Simple Red Blood Cell Lysis Method for the Establishment of B Lymphoblastoid Cell Lines
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Cited by 1 •
2017
A simple red blood cell lysis method for establishing B-LCLs was developed with high immortalization efficiency, requiring a small amount of blood and saving time from initiation to cryopreservation.
03:12
A Procedure for Bacterial Harvesting and Mechanical Lysis
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2025
Source: Mercier, N., et al. MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria. J. Vis. Exp. (2021)This video demonstrates a step-by-step procedure for harvesting bacterial cells and mechanically lysing them to release intracellular contents.
04:11
Isolation of Endospores from an Environmental Sample by Targeted Lysis of Vegetative Cells
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2025
Source: Wunderlin, T., et al. Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells. J. Vis. Exp. (2016)This video demonstrates a method to isolate endospores from an environmental sample containing both vegetative cells and various bacteria. The procedure involves filtration, mechanical disruption, and selective chemical treatment to weaken and lyse vegetative cells while preserving endospores. The final step isolates the resilient endospores through...
09:53
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
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Cited by 4 •
2009
An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.
FAQs
Selection depends on the sample type, the intracellular material being recovered, and the need to preserve that material for later analysis. Cultured cells, tissues, and microorganisms may respond differently to mechanical force, osmotic pressure, detergents, or enzymes. A suitable approach balances effective disruption with limited degradation, improving recovery of proteins, nucleic acids, organelles, or other target molecules.
These treatments weaken cellular barriers through different types of stress. Mechanical force physically disrupts the cell, osmotic pressure promotes damage through an imbalance across the barrier, and detergents alter membrane structure. Enzymes weaken cellular components through biochemical action. Because each approach acts differently, researchers can select one treatment or combine several to match the sample and target.
Combining treatments can address different protective features of a cell or sample. For example, mechanical force may provide physical disruption while detergents, enzymes, or osmotic conditions further weaken cellular barriers. This combined strategy can increase release of intracellular contents, but conditions still need to be controlled so the desired proteins, nucleic acids, organelles, or other molecules remain suitable for analysis.
The conditions determine whether intracellular contents are released efficiently and whether target molecules remain intact afterward. Insufficient disruption can reduce recovery, whereas overly harsh or poorly selected treatment may compromise the material needed for analysis. Researchers therefore adjust the method and conditions to balance release with preservation before applying downstream procedures such as electrophoresis, PCR, sequencing, or biochemical assays.
A typical workflow begins by identifying the sample and the intracellular material of interest, followed by choosing a suitable disruption strategy. Mechanical force, osmotic pressure, detergents, enzymes, or a combination are then applied under selected conditions. The resulting cell contents can be prepared for separation or analysis, including centrifugation, electrophoresis, PCR, sequencing, or biochemical assays.
The principal options are mechanical force, osmotic pressure, detergents, and enzymes. Their use depends on the cellular sample and the molecules or structures that must be recovered. Researchers may apply one approach or combine treatments to improve disruption. Choosing among these options helps prepare samples containing proteins, nucleic acids, organelles, or other intracellular components for subsequent study.
Lysed samples provide access to intracellular proteins, nucleic acids, organelles, and other biomolecules that are not readily studied within intact cells. Once released, these contents can support centrifugation, electrophoresis, PCR, sequencing, and biochemical assays. The quality of the preparation influences how reliably those techniques can examine composition, structure, or biological activity.
The approach supports analysis of diverse biological materials, including cultured cells, tissues, and microorganisms. It connects the physical disruption of cellular barriers with the recovery of molecules and organelles needed for laboratory investigation. Because different targets require different preservation priorities, selecting suitable treatments and conditions is central to obtaining samples that work effectively in molecular and biochemical studies.