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Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
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Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

DOI:
10.3791/1656-v

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09:53 min

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December 31, 2009

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,
1Department of Mechanical Engineering,University of Louisville, 2Department of Bioengineering,University of Louisville

Chapters

  • 00:00Title
  • 00:40Introduction
  • 01:13Priming the Microfluidic Device
  • 02:39Preparing the Syringe Pumps
  • 05:01Erythrocyte Lysis
  • 08:32Representative Results/ Outcome
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.

Tags

Automated Microfluidic Blood LysisCirculating Nucleated CellsCNCs EnrichmentMicrofluidic ProtocolDevice FabricationSample CollectionDevice SetupBlood LysisErythrocyte LysisHerringbone StructuresIsotonic ConditionsParaformaldehyde FixationFlow Cytometry AnalysisDisease Pathogenesis Analysis

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