Cryogenic Vials
Cryogenic Vials - Related Videos
09:50
Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures
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Cited by 4 •
2017
A protocol for determining the vitreous phase densities of micro- to pico-liter size drops of aqueous mixtures at cryogenic temperatures is described.
02:03
Preparation of Legionella pneumophila for Cryogenic Electron Tomography
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2025
Source: Chetrit, D., et al. Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System. J. Vis. Exp. (2020)The video describes how to prepare Legionella pneumophila samples for cryo-electron tomography by combining bacterial culture, gold nanoparticle marking, and vitrification techniques. The process preserves native cellular structures and enables high-resolution imaging of membrane-associated secretion...
05:47
Do-It-Yourself Device for Recovery of Cryopreserved Samples Accidentally Dropped into Cryogenic Storage Tanks
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Cited by 5 •
2012
Here we present a low cost, durable cryotolerant device for sample retrieval from Dewar tanks filled with liquid nitrogen. The ease of construction and modular design of the device makes the process of sample retrieval from cryogenic tanks safe and easy.
08:29
Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
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Cited by 2 •
2025
This workflow enables lamella production targeting fluorescently labeled biological structures that are small (<1 μm in axial extent) and rare (1 copy per cell) using a cryogenic tri-coincident imaging platform. This platform integrates fluorescence microscopy, focused ion beam milling, and scanning electron microscopy at a single focal position and enables simultaneous fluorescence microscopy while milling.
06:32
Optimizing Sample Preparation for Cryogenic Electron Microscopy
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2025
This protocol presents a practical method using Methanocaldococcus jannaschii (MjsHSP16.5) as an example to address uneven particle distribution through sample preparation optimization, providing a reference for researchers to efficiently elucidate macromolecular structures using cryogenic electron microscopy (cryo-EM).
FAQs
Cryoprotectants are added before cooling to reduce damage associated with ice crystal formation. Their role complements controlled cooling rather than replacing it: the sample must still be cooled under managed conditions and transferred to ultra-low-temperature storage. Together, these measures help preserve both cellular viability and molecular properties during long-term biological sample banking.
Controlled cooling helps limit the damage that can occur as biological samples transition to ultra-low temperatures. This step helps reduce ice crystal formation, which can compromise preserved material. Applying a defined cooling process before placement in nitrogen vapor or liquid-phase storage therefore supports the continued viability and molecular integrity of cells, tissues, microorganisms, and other specimens.
Nitrogen vapor and liquid phases provide ultra-low-temperature environments for storing biological specimens after controlled cooling. These conditions limit metabolic activity, helping preserve samples over extended periods. The choice of storage phase should be managed alongside reliable sealing and temperature control, because maintaining stable conditions is important for protecting viability and molecular properties during storage.
Before storage, researchers combine the biological sample with suitable cryoprotectants, cool it under controlled conditions, and place it in an ultra-low-temperature nitrogen environment. The vial must also be labeled reliably and sealed securely. Managing these steps together reduces risks to sample identity, containment, and preservation, supporting later use in cell culture, disease studies, or biotechnology.
Cryogenic vials can support banking of cell lines, primary cells, tissues, microorganisms, and other research samples. This breadth makes them useful across biological workflows rather than only for one specimen type. Depending on the study, preserved materials can contribute to cell culture, disease research, biotechnology, and other laboratory investigations requiring access to stored specimens.
Reliable labeling helps maintain sample identity, while secure sealing supports containment during ultra-low-temperature storage. Consistent temperature management helps preserve the intended storage conditions and limits risks to viability and molecular properties. Together, these practices strengthen sample integrity and support reproducible laboratory research, especially when stored materials are later used for cell culture, disease studies, or biotechnology.