Dephosphorylation
Dephosphorylation - Related Videos
02:54
Protein Kinases and Phosphatases
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2020
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven. Protein kinases Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
03:40
Evaluation of Stromal Cytokine-Induced Intracellular Protein Phosphorylation Using Phospho-Flow Cytometry
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2025
This video demonstrates the evaluation of stromal cytokine-induced intracellular phosphorylation of proteins in leukemia cells. These intracellular phosphorylated proteins are quantified using the phospho-flow cytometry technique.
03:03
Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies
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2025
This video demonstrates an assay measuring the antibody-mediated activation of T cell effector functions. Adding an influenza virus hemagglutinin (HA)-specific monoclonal antibody to transfected mammalian cells expressing HA results in the formation of immune complexes with HA. Upon introducing engineered T cells expressing Fc receptors and a luciferase reporter, the T cells are activated by the antibody-HA complexes, and this activation is detected by adding a luciferase substrate and...
02:57
Transfecting Primary Macrophages with Modified mRNA
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2025
This video demonstrates the process of transfecting primary macrophages with modified mRNA encoding green fluorescent protein. These modified mRNAs, designed to reduce immunogenicity and improve its stability, ensure the successful transfection and subsequent expression of green fluorescent proteins, which is confirmed through fluorescence microscopy.
04:06
Performing Double Fluorescence In Situ Hybridization to Detect Gene Expression in Mouse Brain Sections
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2025
Source: Jolly, S., et.al. Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections. J. Vis. Exp. (2016).This video demonstrates the use of double fluorescence in situ hybridization to visualize the expression of two RNA targets in mouse brain sections. The protocol involves sequential detection of RNA using probes labeled with fluorescein isothiocyanate and digoxigenin, followed by enzymatic signal...
FAQs
The key chemical event is hydrolysis of a phosphate ester or anhydride bond. Phosphatases use water to cleave that bond, releasing inorganic phosphate and leaving the substrate in a dephosphorylated state. The bond type identifies the chemical linkage being reversed, connecting molecular chemistry with changes in cellular regulation.
Removing a phosphate group can alter a protein’s conformation, activity, localization, or interactions with other molecules. These changes determine how the protein participates in cellular pathways rather than simply removing a chemical group. Consequently, phosphatase activity can redirect signaling, modify protein partnerships, or change where a protein functions.
Dephosphorylation reverses phosphorylation, allowing cells to regulate molecular states in a reversible manner. This opposing relationship gives cellular systems a way to adjust protein behavior and chemical energy rather than maintaining a single fixed state. The balance between the two processes supports controlled responses to stimuli and helps preserve physiological balance.
Examining dephosphorylation can show how cells coordinate signal transduction, metabolism, cell-cycle progression, and gene regulation. Researchers can relate phosphate removal to changes in protein activity, conformation, localization, or interactions, then use those relationships to understand how a stimulus produces a coordinated cellular response.
Its regulatory effects extend across several major biological processes, including signal transduction, metabolism, cell-cycle progression, and gene regulation. In each context, changing the phosphorylation state of relevant molecules can modify their activity or interactions. This makes dephosphorylation important for understanding how cells organize responses and maintain physiological balance.
Phosphatases are important because their activity can influence multiple cellular processes through changes in phosphorylation state. Studying these enzymes helps researchers connect molecular regulation with signaling, metabolism, cell-cycle control, and gene regulation. Their regulatory importance also makes phosphatases targets for biomedical research and the development of potential therapeutic strategies.