Gene Editing
Gene Editing - Related Videos
02:30
A CRISPR-Cas9 Technique for Gene Editing in T Cells
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2025
This video demonstrates an assay for performing gene editing in human T cells using the CRISPR-Cas9 technology. A mixture of primary CD4+ and CD8+ T cells is combined with a CRISPR-Cas9 ribonucleoprotein complex, targeting specific genes for knockout. Upon electroporation, the sgRNA guides Cas9 to the target DNA sequence, creating precise cuts. These cuts are then repaired by the cell's non-homologous end-joining mechanism, leading to gene knockout.
06:57
In Planta Gene Expression and Gene Editing in Moso Bamboo Leaves
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Cited by 3 •
2023
In this study, a novel in planta gene expression and gene editing method mediated by Agrobacterium was developed in bamboo. This method greatly improved the efficiency of gene function validation in bamboo, which has significant implications for accelerating the process of bamboo breeding.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
03:29
PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System
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2025
This video demonstrates the procedure to generate motor neurons by transfection of human iPSCs with PiggyBac transposon system by the ectopic expression of lineage-specific transcription factors.
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
FAQs
The repair pathway strongly influences the result. Nonhomologous end joining reconnects the DNA after a targeted break and can disrupt or remove genetic information. Homology-directed repair instead supports replacement of genetic information when the experiment is designed for that outcome. This distinction lets researchers connect the same targeting strategy to different functional questions.
Programmable nuclease recognition determines where the DNA change is initiated. By directing a CRISPR-Cas system toward a selected sequence, researchers can investigate the function of that region rather than alter DNA randomly. The approach is precise and flexible, but recognition is not a guarantee of perfect specificity; off-target changes must therefore be considered when interpreting experimental results.
Reliability depends on more than selecting a DNA sequence. Delivery challenges can prevent the editing machinery from reaching the relevant biological system, while off-target changes can introduce unintended alterations. Because repair outcomes and unintended effects influence what researchers observe, experimental conclusions should distinguish the intended genetic change from limitations that could affect interpretation.
An experimental workflow begins by selecting a DNA sequence linked to the biological question and using a programmable nuclease system to recognize it. Researchers then deliver the editing components, create the targeted break, and rely on cellular repair. The resulting disruption, removal, or replacement is interpreted alongside possible off-target changes and delivery limitations.
In functional genomics, deliberate changes to selected genes allow researchers to test how those genes contribute to biology. Disease modeling applies the same experimental logic to investigate how genetic changes affect disease-related biology. These uses turn DNA modification into a way to connect a specific genetic alteration with biological function and clarify how variation influences biological systems.
Crop improvement focuses on applying genetic changes to crop biology, whereas gene-based treatments seek to use genetic modification in treatment development. In both settings, precision and flexibility expand experimental possibilities, but delivery, off-target changes, and the consequences of the edit remain important when researchers judge feasibility, potential benefit, and biological outcomes.
Ethical considerations matter because technical precision does not settle whether a proposed application is appropriate. In biology, researchers must evaluate future uses alongside off-target changes, delivery challenges, and intended effects. This broader assessment becomes especially important when moving from experimental possibilities toward potential gene-based treatments or other applications involving deliberate genetic changes in organisms.