Grana

Grana are stacks of flattened thylakoid membranes within chloroplasts that organize the light-dependent reactions of photosynthesis. Their membranes contain chlorophyll, photosystems, electron carriers, and ATP synthase; when pigments absorb light, electrons move through an electron transport chain that builds a proton gradient, driving ATP production while contributing to NADPH formation. By concentrating these components, grana support efficient conversion of light energy into chemical energy, which powers carbon fixation in the chloroplast stroma. Studying grana helps explain plant and algal energy metabolism, chloroplast structure, photosynthetic adaptation, and efforts to improve photosynthetic efficiency.

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Education

JoVE Core - Biology

Anatomy of Chloroplasts

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2019

Green algae and plants, including green stems and unripe fruit, harbor chloroplasts—the vital organelles where photosynthesis takes place. In plants, the highest density of chloroplasts is found in the mesophyll cells of leaves. A double membrane surrounds chloroplasts. The outer membrane faces the cytoplasm of the plant cell on one side and the intermembrane space of the chloroplast on the other. The inner membrane separates the narrow intermembrane space from the aqueous interior of the...

Research

JoVE Journal - Biology

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

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Cited by 19 •

2016

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.

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