Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

Dana Charuvi; Reinat Nevo; Ifat Kaplan-Ashiri; Eyal Shimoni; Ziv Reich

doi:10.3791/54066

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Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

$Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy$

JoVE Journal
Biology

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JoVE Journal Biology

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

12,165 Views

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13:52 min

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June 23, 2016

DOI:

10.3791/54066-v

DOI:

10.3791/54066-v

•

13:52 min

June 23, 2016

•

12149 Views

Dana Charuvi^1,3, Reinat Nevo¹, Ifat Kaplan-Ashiri², Eyal Shimoni², Ziv Reich¹

¹Department of Biological Chemistry,Weizmann Institute of Science ²Department of Chemical Research Support,Weizmann Institute of Science ³Institute of Plant Sciences,Agricultural Research Organization, Volcani Center

Summary

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.