High-pressure Homogenizer
High-pressure Homogenizer - Related Videos
07:26
Synthesis and Microdiffraction at Extreme Pressures and Temperatures
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Cited by 5 •
2013
The laser heated diamond anvil cell combined with synchrotron micro-diffraction techniques allows researchers to explore the nature and properties of new phases of matter at extreme pressure and temperature (PT) conditions. Heterogeneous samples can be characterized in situ under high pressure by 2D mapping and combined powder, single-crystal and multigrain diffraction approaches.
01:23
Test for Homogeneity
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2023
The goodness–of–fit test can be used to decide whether a population fits a given distribution, but it will not suffice to decide whether two populations follow the same unknown distribution. A different test, called the test for homogeneity, can be used to conclude whether two populations have the same distribution. To calculate the test statistic for a test for homogeneity, follow the same procedure as with the test of independence. The hypotheses for the test for homogeneity can be stated as...
02:15
Homogeneous Equilibria for Gaseous Reactions
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2020
Homogeneous Equilibria for Gaseous Reactions For gas-phase reactions, the equilibrium constant may be expressed in terms of either the molar concentrations (Kc) or partial pressures (Kp) of the reactants and products. A relation between these two K values may be simply derived from the ideal gas equation and the definition of molarity. According to the ideal gas equation: Molar concentration or molarity is given by number of moles divided by the volume: Thus, where P is partial pressure, V...
02:36
Isolation of Mosquito-Associated Virus from Mosquito Homogenate
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2026
Source: Huang, D., et al. Mosquito-Associated Virus Isolation from Field-Collected Mosquitoes. J. Vis. Exp. (2022).This video demonstrates the step-by-step procedure for the in vitro isolation of mosquito-associated viruses from mosquito homogenates using fibroblast-like mammalian cells.
08:01
Preparation of Homogeneous MALDI Samples for Quantitative Applications
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Cited by 8 •
2016
A protocol for reducing spatial heterogeneities of ion signals in MALDI mass spectrometry by regulating substrate temperature during sample drying processes is demonstrated.
FAQs
The homogenization valve concentrates the fluid flow through a narrow opening, creating a steep pressure drop as material exits. This rapid change produces shear and turbulence, while cavitation may also contribute to disruption. Together, these forces can break cellular structures or fragment dispersed material, helping convert a heterogeneous sample into a more uniform suspension.
Pressure is a central adjustment because it influences how effectively the sample is disrupted and how much stress the material experiences. Stronger processing conditions may help when cells or dispersed particles resist breakdown, whereas gentler settings can better protect sensitive biomolecules. Researchers therefore select pressure according to the desired balance between uniformity, lysis, and molecular preservation.
Temperature and processing cycles provide additional control over sample quality. Repeated or extended processing can be selected when more complete disruption or dispersion is needed, but biological preparations may require conditions that limit damage to sensitive components. Controlling these variables helps researchers optimize the final suspension rather than treating disruption efficiency as the only experimental endpoint.
A researcher introduces the biological fluid into the instrument, selects pressure and temperature conditions, and applies the intended number of processing cycles through the homogenization valve. The resulting suspension can then support downstream sample preparation, intracellular component recovery, or formulation. Adjusting the operating conditions allows the workflow to match the sample’s sensitivity and desired degree of uniformity.
This approach is useful when researchers need to disrupt microbial or mammalian cells so that intracellular components become available for recovery. The applied shear, turbulence, and possible cavitation act on cellular structures within the flowing sample. Pressure and cycle control are important because the process must provide sufficient lysis while helping preserve sensitive biomolecules for subsequent biological work.
For formulations, the process breaks up dispersed phases as material passes through the valve, producing a more uniform distribution of droplets or particles. This supports emulsion preparation and the production of nanoscale or submicron dispersions. Researchers can regulate pressure, temperature, and processing cycles to balance the desired particle or droplet reduction with preservation of formulation components.