Perforated Carbon Film
Perforated Carbon Film - Related Videos
04:43
Induction of a Subarachnoid Hemorrhage in a Mouse Model through Endovascular Perforation
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2025
The video demonstrates the induction of subarachnoid hemorrhage via endovascular perforation in a mouse model. An incision is made in the neck region of the anesthetized mouse to access the common carotid artery and its bifurcation. A filament is inserted through the arteries to perforate an intracranial artery branch, inducing subarachnoid hemorrhage.
07:58
Electroplating of Thin Films
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2023
Source: Logan G. Kiefer, Andrew R. Falkowski, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Electroplating is a process that uses electric current to reduce dissolved metal cations so that they form a thin coating on an electrode. Other thin film deposition techniques include chemical vapor deposition (CVD), spin coating, dip coating, and sputter deposition among others. CVD uses a gas-phase precursor of the element to be...
01:22
Esophageal Perforation-I: Introduction
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2025
Esophageal perforation is a severe medical condition characterized by a breach in the integrity of the esophageal wall. This breach can occur due to various factors such as trauma, medical procedures, or underlying diseases. When the esophageal wall is compromised, it allows food, fluids, and digestive juices into the chest cavity or adjacent structures, leading to potential complications and health risks. The location of esophageal perforation can vary, occurring anywhere along the esophagus.
10:44
Preparation of Carbon Nanosheets at Room Temperature
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2016
We present the synthesis of an amphiphilic hexayne and its use in the preparation of carbon nanosheets at the air-water interface from a self-assembled monolayer of these reactive, carbon-rich molecular precursors.
02:50
Perforated Patch-Clamp Recordings of Inner Ear Sensory Neuron Cell Bodies
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2025
This video demonstrates the patch-clamp technique for measuring ionic currents in inner ear sensory neuron cell bodies. It details the preparation and use of a polished patch-clamp pipette filled with an internal solution containing amphotericin B, which perforates the membrane to allow ion movement while preserving intracellular conditions. Voltage pulses are applied to trigger and record ion channel activity, providing insights into their properties and behavior.
FAQs
Hole geometry and surface treatment can influence both where specimens collect and how consistently they appear for imaging. Smaller or more widely spaced openings may change the available placement area, while treatment affects specimen distribution across that area. Because these variables shape particle or thin-layer positioning, optimizing them helps produce fields with useful specimen coverage and imaging quality.
The surrounding carbon performs two complementary functions during electron microscopy. It mechanically supports the specimen so material can be held across the patterned film, while its presence around the openings helps reduce background in the electron beam. This arrangement balances specimen stability with a comparatively clearer signal from particles or thin vitrified layers during imaging.
In cryo-electron microscopy, particles suspended in the holes can be imaged within thin vitrified layers. This positioning enables researchers to collect images of biological particles and use those images to determine molecular structures. The openings provide access to specimen views, while the surrounding film maintains support around the vitrified material during transmission electron microscopy.
During preparation, researchers position biological particles or thin vitrified layers across the film’s openings, using the surrounding carbon as support. They consider the pattern’s hole size and spacing, along with any surface treatment, because these features influence specimen distribution. The resulting arrangement determines whether the imaging area contains suitably placed material for transmission electron microscopy.
Perforated carbon film is useful when biological imaging requires specimen support while limiting background in the electron beam. In biology, applications include examining viruses, proteins, membranes, and cellular structures by transmission electron microscopy. The same support format is especially relevant to cryo-electron microscopy, where specimens in the holes can contribute images for structural analysis.
The film can support two related types of information: morphology, meaning the observed form of biological specimens, and, in cryo-electron microscopy, molecular structural information derived from images of particles suspended in the holes. Researchers can therefore use the resulting images to assess visible organization and, in suitable cryo-electron microscopy studies, determine molecular structures.